Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4714_RS04630 Genome accession   NZ_CP137099
Coordinates   838584..839525 (-) Length   313 a.a.
NCBI ID   WP_000249552.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 20824-4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 833584..844525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4714_RS04600 - 833802..834077 (-) 276 WP_050273957.1 hypothetical protein -
  R4714_RS04605 - 834232..835020 (+) 789 WP_050217143.1 LexA family transcriptional regulator -
  R4714_RS04610 - 835022..835786 (+) 765 WP_050110914.1 hypothetical protein -
  R4714_RS04615 - 836093..837538 (+) 1446 WP_050138508.1 recombinase family protein -
  R4714_RS04625 comGB/cglB 837620..838636 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4714_RS04630 comGA/cglA/cilD 838584..839525 (-) 942 WP_000249552.1 competence type IV pilus ATPase ComGA Machinery gene
  R4714_RS04635 - 839601..839966 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4714_RS04640 - 840117..841175 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R4714_RS04645 nagA 841338..842489 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4714_RS04650 - 842642..844459 (-) 1818 WP_050210983.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35587.57 Da        Isoelectric Point: 6.0083

>NTDB_id=818322 R4714_RS04630 WP_000249552.1 838584..839525(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 20824-4]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDIETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=818322 R4714_RS04630 WP_000249552.1 838584..839525(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 20824-4]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGCTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCCAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGTCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACATCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae D39

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae R6

98.403

100

0.984
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

98.403

100

0.984
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comGA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comGA Streptococcus mutans UA159

66.238

99.361

0.658
  comGA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

55.128

99.681

0.55
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364