Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R4535_RS12370 Genome accession   NZ_CP137091
Coordinates   2740599..2741786 (-) Length   395 a.a.
NCBI ID   WP_076024892.1    Uniprot ID   -
Organism   Vibrio cholerae strain DL4211     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2735599..2746786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4535_RS12350 (R4535_12325) sthA 2736263..2737663 (+) 1401 WP_000529898.1 Si-specific NAD(P)(+) transhydrogenase -
  R4535_RS12355 (R4535_12330) rpoH 2737864..2738724 (-) 861 WP_000184359.1 RNA polymerase sigma factor RpoH -
  R4535_RS12360 (R4535_12335) ftsX 2738894..2739886 (-) 993 WP_000279341.1 permease-like cell division protein FtsX -
  R4535_RS12365 (R4535_12340) ftsE 2739876..2740550 (-) 675 WP_000617864.1 cell division ATP-binding protein FtsE -
  R4535_RS12370 (R4535_12345) pilA 2740599..2741786 (-) 1188 WP_076024892.1 signal recognition particle-docking protein FtsY Machinery gene
  R4535_RS12375 (R4535_12350) rsmD 2741990..2742589 (+) 600 WP_001189766.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  R4535_RS12380 (R4535_12355) - 2742586..2742852 (+) 267 WP_000645840.1 DUF1145 domain-containing protein -
  R4535_RS12385 (R4535_12360) - 2742891..2743520 (-) 630 WP_000062276.1 lysoplasmalogenase -
  R4535_RS12390 (R4535_12365) - 2743637..2743867 (-) 231 Protein_2348 hypothetical protein -
  R4535_RS12395 (R4535_12370) - 2744397..2744765 (-) 369 WP_001289435.1 DUF4145 domain-containing protein -
  R4535_RS12400 (R4535_12375) - 2744970..2745299 (+) 330 WP_001117042.1 DUF2500 domain-containing protein -
  R4535_RS12405 (R4535_12380) - 2745469..2745939 (+) 471 WP_000224703.1 Dps family protein -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 43206.88 Da        Isoelectric Point: 4.4258

>NTDB_id=818263 R4535_RS12370 WP_076024892.1 2740599..2741786(-) (pilA) [Vibrio cholerae strain DL4211]
MTEKKKRGLLSWLGFGDEEKSQTTATDTQTQDEPQSQADVEAVAIQAETALTETETETETETETETETETETETATVEPA
RIVEQEKPTESFFARLKRSLSRTKANIGAGFFGLFKGKKIDDDLFEELEEQLLIADVGMDTTSKIIANLTAKASRQQLRD
GEALYGLLKEEMAEILSQVEQPLVIDTEKKPYVILMVGVNGVGKTTTIGKLAKQFQAQGKKVMLAAGDTFRAAAVEQLQV
WGERNQVPVIAQHTGADSASVIYDAIEAAKARGIDVVIADTAGRLQNKSNLMEELRKIVRVMKKIDDSAPHEIMLTLDAG
TGQNAISQAKLFSDVAPITGITLTKLDGTAKGGVIFAIADQFQIPIRYIGVGEKIDDLRPFATQEFIDALFSREE

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=818263 R4535_RS12370 WP_076024892.1 2740599..2741786(-) (pilA) [Vibrio cholerae strain DL4211]
ATGACGGAAAAAAAGAAGCGTGGCTTGTTGTCATGGCTTGGATTTGGTGACGAAGAGAAAAGCCAAACCACTGCAACGGA
TACTCAAACGCAGGATGAACCACAAAGCCAAGCCGATGTTGAAGCGGTAGCGATTCAAGCGGAAACTGCGTTAACAGAAA
CAGAAACAGAAACAGAAACAGAAACAGAAACAGAAACAGAAACAGAAACAGAAACAGAAACAGCCACGGTTGAACCTGCA
CGTATTGTTGAACAAGAAAAACCGACCGAAAGTTTCTTTGCGCGCTTAAAGCGCAGTTTGAGTCGCACCAAAGCGAATAT
TGGCGCAGGCTTCTTTGGCCTGTTTAAAGGCAAAAAAATTGATGATGACCTGTTCGAAGAGCTCGAAGAACAACTGCTGA
TTGCCGATGTCGGCATGGATACGACCAGCAAAATTATCGCTAATTTGACCGCCAAAGCTTCTCGCCAGCAACTGCGTGAT
GGGGAAGCGCTGTACGGTCTGCTGAAAGAAGAGATGGCCGAGATTCTCTCTCAGGTCGAGCAGCCATTGGTTATCGATAC
TGAGAAAAAACCTTACGTGATCTTGATGGTGGGCGTGAATGGGGTGGGGAAAACCACCACGATCGGTAAACTGGCTAAAC
AGTTCCAAGCGCAAGGCAAGAAAGTGATGTTGGCAGCGGGCGATACCTTCCGTGCTGCAGCGGTTGAGCAGTTGCAAGTG
TGGGGAGAGCGCAATCAAGTGCCTGTCATCGCCCAGCATACTGGCGCCGACAGTGCCTCCGTCATTTATGATGCGATTGA
AGCGGCGAAAGCGCGCGGGATTGACGTGGTGATTGCGGATACCGCAGGCCGTCTGCAAAACAAAAGTAACCTGATGGAAG
AGCTGCGCAAGATTGTGCGCGTGATGAAGAAGATTGATGACAGCGCGCCACATGAAATCATGTTGACGCTGGATGCGGGT
ACCGGACAAAACGCGATCAGTCAGGCCAAATTGTTTAGTGATGTAGCGCCCATCACAGGCATTACCTTAACTAAGCTTGA
TGGCACCGCTAAAGGTGGCGTGATTTTCGCGATTGCCGATCAATTCCAGATCCCAATCCGTTATATCGGTGTCGGCGAGA
AAATTGACGACTTACGTCCATTTGCAACACAAGAGTTTATCGACGCGCTATTTAGCCGCGAAGAATAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(192-391)

SRP54-type protein, helical bundle domain (SRP54_N)

(111-172)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.706

86.076

0.428