Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   R3J30_RS07445 Genome accession   NZ_CP136980
Coordinates   1593490..1594875 (+) Length   461 a.a.
NCBI ID   WP_031345738.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain CFBP8417     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1588490..1599875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J30_RS07415 (R3J30_07405) - 1589790..1590017 (-) 228 WP_004083771.1 BolA family protein -
  R3J30_RS07420 (R3J30_07410) - 1590079..1591080 (+) 1002 WP_027700003.1 KpsF/GutQ family sugar-phosphate isomerase -
  R3J30_RS07425 (R3J30_07415) - 1591098..1591646 (+) 549 WP_004083773.1 HAD family hydrolase -
  R3J30_RS07430 (R3J30_07420) lptC 1591643..1592212 (+) 570 WP_004083774.1 LPS export ABC transporter periplasmic protein LptC -
  R3J30_RS07435 (R3J30_07425) lptA 1592190..1592720 (+) 531 WP_004083775.1 lipopolysaccharide transport periplasmic protein LptA -
  R3J30_RS07440 (R3J30_07430) lptB 1592720..1593442 (+) 723 WP_004083776.1 LPS export ABC transporter ATP-binding protein -
  R3J30_RS07445 (R3J30_07435) rpoN/rpoN1 1593490..1594875 (+) 1386 WP_031345738.1 RNA polymerase factor sigma-54 Machinery gene
  R3J30_RS07450 (R3J30_07440) hpf 1594909..1595226 (+) 318 WP_004083778.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  R3J30_RS07455 (R3J30_07445) hprK 1595492..1596442 (+) 951 WP_004083779.1 HPr(Ser) kinase/phosphatase -
  R3J30_RS07460 (R3J30_07450) rapZ 1596439..1597311 (+) 873 WP_004083780.1 RNase adapter RapZ -
  R3J30_RS07465 (R3J30_07455) - 1597682..1598074 (+) 393 WP_004083781.1 PTS sugar transporter subunit IIA -
  R3J30_RS07470 (R3J30_07460) - 1598067..1598336 (+) 270 WP_004083782.1 HPr family phosphocarrier protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 51226.22 Da        Isoelectric Point: 6.3573

>NTDB_id=817244 R3J30_RS07445 WP_031345738.1 1593490..1594875(+) (rpoN/rpoN1) [Xylella fastidiosa subsp. multiplex strain CFBP8417]
MKARLRTSIGQQLVMTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEAAETEVETEDWQAKESAWDIN
GSKSNENDDNNPAERLTNENTLTDHLLWQLRLSPLSARDYQIGTVLIDAIEEDGYLREPLSTLTATITPLSVDESELLPV
LRHIQSFDPTGVAARSLAECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRGLD
PRPGKKIGDLEVGTYVIPDCVIWRQHGLWQATLLGHGFPQVVLHRGYEQLINRCNDANASYLRNQLQEARWLLKGLEARG
ETLLKVVNSLIRHQAGFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVRTPRGTLALRTFFASGISTDNGGETSS
SAIQAIIRRLIETENPRKPLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVLG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=817244 R3J30_RS07445 WP_031345738.1 1593490..1594875(+) (rpoN/rpoN1) [Xylella fastidiosa subsp. multiplex strain CFBP8417]
TTGAAAGCAAGACTGCGAACATCCATCGGACAACAGCTGGTCATGACACCCCAGCTACACCAAAGCATCCGTCTGCTGCA
AATGTCCAACGCCGAATTGCAACTGGAAATCACCGCAGCGATAGAGAGCAATCCACTCTTGGACTGGGCCGAGAACCAAT
CATCGGAGACCAGCGAAGCTGCCGAAACGGAAGTAGAAACAGAAGATTGGCAGGCCAAGGAGTCGGCCTGGGATATCAAC
GGCAGTAAAAGCAACGAAAATGACGACAACAACCCTGCTGAACGTCTGACCAACGAAAACACTCTCACTGATCACTTGCT
GTGGCAACTGCGCCTCTCTCCACTGTCAGCACGTGATTACCAAATAGGCACCGTCTTGATTGATGCCATAGAAGAAGATG
GTTACCTGCGTGAACCTTTATCCACACTGACTGCAACCATAACCCCGTTATCTGTAGATGAAAGCGAACTGCTACCAGTA
TTACGACACATTCAATCTTTTGACCCGACCGGCGTAGCTGCACGCTCGCTGGCCGAGTGCTTGACGCTGCAACTGAAATC
ATTGAGAGAAGCGATACCCGGACGCACACTTGCACTGCAGATTGTCAACAGCCCACTGCTCGAACAATTGCCCCGCAGTG
GTGTCACCGGACTGGCGCGCGAACTGAAACGACCCTCCCAAGACGTCCAACAGGCAGTGGAATTGATCCGTGGACTGGAC
CCTCGCCCAGGTAAGAAAATTGGCGACTTAGAAGTTGGAACCTACGTTATACCGGACTGTGTGATCTGGCGCCAACATGG
GCTGTGGCAAGCTACGCTGTTGGGACATGGATTCCCACAAGTCGTCCTACACCGCGGCTACGAACAGCTCATCAACCGCT
GCAATGACGCCAATGCGAGCTACCTACGCAACCAACTCCAAGAAGCACGATGGCTGCTCAAGGGCCTGGAAGCACGCGGT
GAAACTTTGTTAAAGGTGGTAAACAGCCTCATACGTCACCAAGCGGGATTCTTGGAATTCGGCCAGCACGCGTTACGTCC
ATTGACTATCCGCGAATTAGCAACGGAACTTGCCCTGCATGAATCCACCGTATCCCGTGCAATTGCTGGCAAGTATGTAC
GCACCCCACGTGGCACCTTAGCGCTACGCACCTTTTTCGCTTCAGGAATCAGCACCGACAACGGCGGAGAGACCTCCAGC
TCTGCCATTCAAGCAATCATCAGACGTCTCATAGAAACTGAAAACCCCCGCAAGCCACTCTCTGACGCCAAACTAGCTGA
ATTGCTGAAGACATCCGGTATTCCAGTAGCGCGACGCACCATAGCAAAGTATCGTGATGCTATGAACATCTCCGCATCCC
ACGAACGCGTCCGAGTCCTAGGATGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(300-458)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(94-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

38.2

100

0.414