Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   R3J35_RS05625 Genome accession   NZ_CP136972
Coordinates   1247211..1248599 (+) Length   462 a.a.
NCBI ID   WP_080654434.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain CFBP8173     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1242211..1253599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J35_RS05595 (R3J35_05585) - 1243517..1243744 (-) 228 WP_004083771.1 BolA family protein -
  R3J35_RS05600 (R3J35_05590) - 1243806..1244807 (+) 1002 WP_027700003.1 KpsF/GutQ family sugar-phosphate isomerase -
  R3J35_RS05605 (R3J35_05595) - 1244825..1245373 (+) 549 WP_004083773.1 HAD family hydrolase -
  R3J35_RS05610 (R3J35_05600) lptC 1245370..1245939 (+) 570 WP_004083774.1 LPS export ABC transporter periplasmic protein LptC -
  R3J35_RS05615 (R3J35_05605) lptA 1245917..1246447 (+) 531 WP_027700004.1 lipopolysaccharide transport periplasmic protein LptA -
  R3J35_RS05620 (R3J35_05610) lptB 1246447..1247169 (+) 723 WP_020850896.1 LPS export ABC transporter ATP-binding protein -
  R3J35_RS05625 (R3J35_05615) rpoN/rpoN1 1247211..1248599 (+) 1389 WP_080654434.1 RNA polymerase factor sigma-54 Machinery gene
  R3J35_RS05630 (R3J35_05620) hpf 1248690..1249007 (+) 318 WP_004083778.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  R3J35_RS05635 (R3J35_05625) hprK 1249273..1250223 (+) 951 WP_004083779.1 HPr(Ser) kinase/phosphatase -
  R3J35_RS05640 (R3J35_05630) rapZ 1250220..1251092 (+) 873 WP_027700006.1 RNase adapter RapZ -
  R3J35_RS05645 (R3J35_05635) - 1251464..1251856 (+) 393 WP_004083781.1 PTS sugar transporter subunit IIA -
  R3J35_RS05650 (R3J35_05640) - 1251849..1252118 (+) 270 WP_004089222.1 HPr family phosphocarrier protein -

Sequence

Protein


Download         Length: 462 a.a.        Molecular weight: 51353.49 Da        Isoelectric Point: 6.3573

>NTDB_id=816943 R3J35_RS05625 WP_080654434.1 1247211..1248599(+) (rpoN/rpoN1) [Xylella fastidiosa subsp. multiplex strain CFBP8173]
MTLKARLRTSIGQQLVMTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEATEREVETEDWQAKESAWD
INGSKSNENDDNNPAERLTTGNTLTDHLLWQLHLSPLSARDYQIGTVLIDAIEEDGYLRELLSTLTATLAPLSVDEIELL
PVLRHIQSFDPSGVAARSLAECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRG
LDPRPGKKIGDLEVGTYVVPDCVIWRQHGLWQAALLERAFPQVVLHRGYEQLINSCNDANASYLRNQLQEARWLLKGLEA
RGETLLKVVNSLIRHQAGFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVRTPRGTLPLRTFFASGISTDSGGET
SSSAIQAIIRRLIETENPRKPLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVL

Nucleotide


Download         Length: 1389 bp        

>NTDB_id=816943 R3J35_RS05625 WP_080654434.1 1247211..1248599(+) (rpoN/rpoN1) [Xylella fastidiosa subsp. multiplex strain CFBP8173]
ATGACATTGAAAGCAAGACTGCGAACATCCATCGGACAACAGCTGGTCATGACACCCCAGCTACACCAAAGCATCCGTCT
GCTGCAAATGTCCAACGCCGAATTGCAACTGGAAATCACCGCCGCGATAGAGAGCAATCCACTCTTGGACTGGGCCGAGA
ACCAATCATCGGAGACCAGCGAAGCTACCGAAAGGGAAGTAGAAACAGAAGATTGGCAAGCCAAAGAATCCGCCTGGGAT
ATCAACGGCAGTAAAAGCAACGAAAATGACGACAACAACCCTGCTGAACGTCTGACCACCGGGAACACTCTCACTGATCA
CTTGCTGTGGCAACTGCACCTCTCCCCACTGTCAGCACGTGATTACCAAATAGGCACCGTCTTGATTGATGCCATAGAAG
AAGATGGTTACCTGCGTGAACTTTTGTCCACACTGACTGCAACCCTGGCCCCGTTATCCGTAGATGAAATCGAACTGCTA
CCAGTATTACGACACATTCAATCTTTTGACCCGAGCGGCGTGGCTGCACGCTCACTGGCCGAGTGCTTGACACTGCAACT
GAAATCATTGAGAGAAGCGATACCCGGACGCACACTTGCACTGCAGATTGTCAACAGCCCACTGCTCGAACAATTGCCGC
GCAGTGGTGTCACCGGACTGGCACGCGAACTGAAACGACCCTCCCAAGACGTCCAACAGGCAGTGGAATTGATCCGTGGA
CTGGACCCTCGCCCAGGTAAGAAAATTGGCGACTTAGAAGTTGGAACCTACGTTGTACCGGACTGTGTGATCTGGCGCCA
ACATGGGTTGTGGCAAGCCGCGTTGTTGGAACGTGCATTCCCACAGGTCGTCCTCCACCGCGGCTACGAACAGCTCATCA
ACAGCTGCAATGACGCCAATGCGAGCTACCTACGCAACCAACTCCAGGAAGCACGGTGGCTGCTCAAGGGCCTGGAAGCA
CGCGGTGAAACCTTGTTAAAAGTGGTAAACAGCCTCATACGTCACCAAGCGGGATTCTTGGAATTCGGCCAACACGCGTT
ACGTCCATTGACTATCCGCGAATTAGCAACGGAACTTGCCCTGCATGAATCCACCGTATCCCGTGCAATTGCTGGCAAGT
ATGTACGCACCCCACGTGGCACCTTACCGCTACGCACCTTTTTCGCTTCAGGAATCAGCACCGACAGCGGCGGAGAGACC
TCCAGCTCTGCCATTCAAGCAATCATCAGACGTCTCATAGAAACTGAAAACCCCCGCAAGCCACTCTCTGACGCCAAACT
AGCTGAATTACTGAAGACATCCGGTATCCCAGTAGCGCGACGCACCATAGCAAAGTATCGTGATGCTATGAACATCTCCG
CATCCCACGAACGCGTCCGAGTCCTCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(7-50)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(96-289)

Sigma-54, DNA binding domain (Sigma54_DBD)

(302-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

38.2

100

0.413