Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilH   Type   Machinery gene
Locus tag   R3J28_RS09005 Genome accession   NZ_CP136971
Coordinates   1929145..1929531 (+) Length   128 a.a.
NCBI ID   WP_004083584.1    Uniprot ID   B2I7W3
Organism   Xylella fastidiosa subsp. multiplex strain Santa29b     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1924145..1934531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J28_RS08985 (R3J28_08955) - 1925559..1925744 (-) 186 WP_004089787.1 DUF2065 family protein -
  R3J28_RS08990 (R3J28_08960) hflC 1925904..1926767 (-) 864 WP_004083581.1 protease modulator HflC -
  R3J28_RS08995 (R3J28_08965) hflK 1926764..1927903 (-) 1140 WP_004083582.1 FtsH protease activity modulator HflK -
  R3J28_RS09000 (R3J28_08970) - 1928048..1929052 (+) 1005 WP_004083583.1 oxidoreductase -
  R3J28_RS09005 (R3J28_08975) pilH 1929145..1929531 (+) 387 WP_004083584.1 twitching motility response regulator PilH Machinery gene
  R3J28_RS09010 (R3J28_08980) - 1930114..1930464 (-) 351 WP_004083585.1 DMT family protein -
  R3J28_RS09015 (R3J28_08985) - 1931546..1931920 (-) 375 WP_004083586.1 H-NS family nucleoid-associated regulatory protein -
  R3J28_RS09020 (R3J28_08990) - 1932066..1933760 (-) 1695 WP_004083587.1 proline--tRNA ligase -
  R3J28_RS09025 (R3J28_08995) - 1933845..1934222 (-) 378 WP_004083588.1 DUF4124 domain-containing protein -

Sequence


Protein


Download         Length: 128 a.a.        Molecular weight: 14261.45 Da        Isoelectric Point: 6.7299

>NTDB_id=816894 R3J28_RS09005 WP_004083584.1 1929145..1929531(+) (pilH) [Xylella fastidiosa subsp. multiplex strain Santa29b]
MARILIVEDSPSQLLSIQRIVEKLGHETITATDGNTGVEAAKAHLPDLVLMDVVMPKINGFQATRTLKREPATQHVPVVL
VTTKDQDTDRLWGIRQGARAYITKPFSESELLEVMERIFSQPKAKANE

Nucleotide


Download         Length: 387 bp        

>NTDB_id=816894 R3J28_RS09005 WP_004083584.1 1929145..1929531(+) (pilH) [Xylella fastidiosa subsp. multiplex strain Santa29b]
ATGGCACGCATTTTGATCGTCGAGGACTCACCGTCACAATTACTCAGCATCCAGAGAATCGTCGAAAAACTAGGGCACGA
AACAATTACCGCAACCGATGGCAACACCGGGGTGGAGGCAGCCAAAGCACATTTACCTGATCTGGTACTGATGGACGTGG
TCATGCCTAAAATTAATGGGTTTCAAGCCACACGCACACTCAAGCGCGAACCAGCAACGCAACATGTACCAGTCGTCCTG
GTAACCACCAAGGACCAAGACACTGATCGGCTATGGGGCATACGCCAGGGCGCGCGCGCCTACATCACCAAGCCTTTCTC
AGAGAGTGAATTGCTAGAAGTCATGGAAAGAATATTCAGCCAACCAAAAGCAAAAGCGAACGAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB B2I7W3

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

41.176

92.969

0.383