Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   R3J31_RS03625 Genome accession   NZ_CP136968
Coordinates   827168..828553 (-) Length   461 a.a.
NCBI ID   WP_031345738.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain XYL1966/18     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 822168..833553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J31_RS03600 (R3J31_03590) - 823707..823976 (-) 270 WP_004083782.1 HPr family phosphocarrier protein -
  R3J31_RS03605 (R3J31_03595) - 823969..824361 (-) 393 WP_004083781.1 PTS sugar transporter subunit IIA -
  R3J31_RS03610 (R3J31_03600) rapZ 824732..825604 (-) 873 WP_004083780.1 RNase adapter RapZ -
  R3J31_RS03615 (R3J31_03605) hprK 825601..826551 (-) 951 WP_004083779.1 HPr(Ser) kinase/phosphatase -
  R3J31_RS03620 (R3J31_03610) hpf 826817..827134 (-) 318 WP_004083778.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  R3J31_RS03625 (R3J31_03615) rpoN/rpoN1 827168..828553 (-) 1386 WP_031345738.1 RNA polymerase factor sigma-54 Machinery gene
  R3J31_RS03630 (R3J31_03620) lptB 828601..829323 (-) 723 WP_004083776.1 LPS export ABC transporter ATP-binding protein -
  R3J31_RS03635 (R3J31_03625) lptA 829323..829853 (-) 531 WP_154128510.1 lipopolysaccharide transport periplasmic protein LptA -
  R3J31_RS03640 (R3J31_03630) lptC 829831..830400 (-) 570 WP_004083774.1 LPS export ABC transporter periplasmic protein LptC -
  R3J31_RS03645 (R3J31_03635) - 830397..830945 (-) 549 WP_004083773.1 HAD family hydrolase -
  R3J31_RS03650 (R3J31_03640) - 830963..831964 (-) 1002 WP_027700003.1 KpsF/GutQ family sugar-phosphate isomerase -
  R3J31_RS03655 (R3J31_03645) - 832026..832253 (+) 228 WP_004083771.1 BolA family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 51226.22 Da        Isoelectric Point: 6.3573

>NTDB_id=816699 R3J31_RS03625 WP_031345738.1 827168..828553(-) (rpoN/rpoN1) [Xylella fastidiosa subsp. multiplex strain XYL1966/18]
MKARLRTSIGQQLVMTPQLHQSIRLLQMSNAELQLEITAAIESNPLLDWAENQSSETSEAAETEVETEDWQAKESAWDIN
GSKSNENDDNNPAERLTNENTLTDHLLWQLRLSPLSARDYQIGTVLIDAIEEDGYLREPLSTLTATITPLSVDESELLPV
LRHIQSFDPTGVAARSLAECLTLQLKSLREAIPGRTLALQIVNSPLLEQLPRSGVTGLARELKRPSQDVQQAVELIRGLD
PRPGKKIGDLEVGTYVIPDCVIWRQHGLWQATLLGHGFPQVVLHRGYEQLINRCNDANASYLRNQLQEARWLLKGLEARG
ETLLKVVNSLIRHQAGFLEFGQHALRPLTIRELATELALHESTVSRAIAGKYVRTPRGTLALRTFFASGISTDNGGETSS
SAIQAIIRRLIETENPRKPLSDAKLAELLKTSGIPVARRTIAKYRDAMNISASHERVRVLG

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=816699 R3J31_RS03625 WP_031345738.1 827168..828553(-) (rpoN/rpoN1) [Xylella fastidiosa subsp. multiplex strain XYL1966/18]
TTGAAAGCAAGACTGCGAACATCCATCGGACAACAGCTGGTCATGACACCCCAGCTACACCAAAGCATCCGTCTGCTGCA
AATGTCCAACGCCGAATTGCAACTGGAAATCACCGCAGCGATAGAGAGCAATCCACTCTTGGACTGGGCCGAGAACCAAT
CATCGGAGACCAGCGAAGCTGCCGAAACGGAAGTAGAAACAGAAGATTGGCAGGCCAAGGAGTCGGCCTGGGATATCAAC
GGCAGTAAAAGCAACGAAAATGACGACAACAACCCTGCTGAACGTCTGACCAACGAAAACACTCTCACTGATCACTTGCT
GTGGCAACTGCGCCTCTCTCCACTGTCAGCACGTGATTACCAAATAGGCACCGTCTTGATTGATGCCATAGAAGAAGATG
GTTACCTGCGTGAACCTTTATCCACACTGACTGCAACCATAACCCCGTTATCTGTAGATGAAAGCGAACTGCTACCAGTA
TTACGACACATTCAATCTTTTGACCCGACCGGCGTAGCTGCACGCTCGCTGGCCGAGTGCTTGACGCTGCAACTGAAATC
ATTGAGAGAAGCGATACCCGGACGCACACTTGCACTGCAGATTGTCAACAGCCCACTGCTCGAACAATTGCCCCGCAGTG
GTGTCACCGGACTGGCGCGCGAACTGAAACGACCCTCCCAAGACGTCCAACAGGCAGTGGAATTGATCCGTGGACTGGAC
CCTCGCCCAGGTAAGAAAATTGGCGACTTAGAAGTTGGAACCTACGTTATACCGGACTGTGTGATCTGGCGCCAACATGG
GCTGTGGCAAGCTACGCTGTTGGGACATGGATTCCCACAAGTCGTCCTACACCGCGGCTACGAACAGCTCATCAACCGCT
GCAATGACGCCAATGCGAGCTACCTACGCAACCAACTCCAAGAAGCACGATGGCTGCTCAAGGGCCTGGAAGCACGCGGT
GAAACTTTGTTAAAGGTGGTAAACAGCCTCATACGTCACCAAGCGGGGTTCTTGGAATTCGGCCAGCACGCGTTACGTCC
ATTGACTATCCGCGAATTAGCAACGGAACTTGCCCTGCATGAATCCACCGTATCCCGTGCAATTGCTGGCAAGTATGTAC
GCACCCCACGTGGCACCTTAGCGCTACGCACCTTTTTCGCTTCAGGAATCAGCACCGACAACGGCGGAGAGACCTCCAGC
TCTGCCATTCAAGCAATCATCAGACGTCTCATAGAAACTGAAAACCCCCGCAAGCCACTCTCTGACGCCAAACTAGCTGA
ATTGCTGAAGACATCCGGTATTCCAGTAGCGCGACGCACCATAGCAAAGTATCGTGATGCTATGAACATCTCCGCATCCC
ACGAACGCGTCCGAGTCCTAGGATGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(300-458)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(94-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

38.2

100

0.414