Detailed information NTG0814929

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QA442_RS16750
• Genome accession: NZ_CP121861
• Coordinates: 3203369..3207073 (-)
• Length: 1234 a.a.
• NCBI ID: WP_095432671.1
• Uniprot ID: -
• Organism: Bacillus stercoris strain Mal05
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 3198369..3212073

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QA442_RS16735 (QA442_16735)	hlpB	3198417..3198719 (-)	303	WP_014663462.1	HNH nuclease-like protein HlpB	-
QA442_RS16740 (QA442_16740)	sbcC	3198733..3202125 (-)	3393	WP_095432669.1	exonuclease subunit SbcC	-
QA442_RS16745 (QA442_16745)	sbcD	3202122..3203297 (-)	1176	WP_095432670.1	exonuclease subunit SbcD	-
QA442_RS16750 (QA442_16750)	addA	3203369..3207073 (-)	3705	WP_095432671.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QA442_RS16755 (QA442_16755)	addB	3207060..3210559 (-)	3500	Protein_3280	helicase-exonuclease AddAB subunit AddB	-
QA442_RS16760 (QA442_16760)	-	3210743..3211180 (+)	438	WP_040083516.1	ferritin family protein	-
QA442_RS16765 (QA442_16765)	-	3211299..3211628 (+)	330	WP_095432673.1	four-helix bundle copper-binding protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141423.65 Da
• Isoelectric Point: 5.0218

>NTDB_id=814929 QA442_RS16750 WP_095432671.1 3203369..3207073(-) (addA) [Bacillus stercoris strain Mal05]
MNIPKPADSTWTDDQWNAIVSTDQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVQRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIEHQDDFSELYRRVPAVSFKRAKAVKGDEFDPALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLTEMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTAQNDKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTGGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAELKLGASYPDNDETETELLLIDNAEDTDANEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLHTFYGHLQKWRVFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKNHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDLEALAGNGTPVHADISGHPARFAVQMIHSYD
LLDDDVEERMEEKSERLEAIRRGEPVPGSFAFDEKAREQLNWTYPHQEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLSHVPSIEEAEQTVQRLYEKELLTEEQKDAIDIEEIVQFFHTEIGG
QLIGAKWKDREIPFSLALPAKEIYPDAEEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYD
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide

• Length: 3705 bp

>NTDB_id=814929 QA442_RS16750 WP_095432671.1 3203369..3207073(-) (addA) [Bacillus stercoris strain Mal05]
ATGAACATTCCTAAACCGGCAGATAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGACCAGGATATTCT
CGTGGCAGCGGCTGCGGGCTCTGGTAAAACCGCTGTGCTCGTTGAACGAATGATTCGGAAAATCACCGCGGAGGAAAACC
CGATTGATGTAGACCGCCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGAATTGCGGATGCC
TTGGAAAAAGAGCTTGTACAACGCCCCGGCTCGCTGCATATTAGACGGCAGCTTTCTCTTTTGAACCGGGCCAGCATTTC
GACGCTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGTTTTCGGATAGCTG
ATCAGACGGAAGGCGAGCTGCTTGGGGATGAAGTGCTTGACGAGCTGTTTGAAGACGAATATGCAAAGGGCGAAAAGGCA
TTTTTTGAGCTTGTTGACCGCTACACGACAGACCGGCACGATCTGGATCTCCAATTTCTCGTTAAACAAGTGTACGAGTA
TTCCCGATCCCATCCCAACCCGGAGGCGTGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCAGAAAAGAGCGCCATCG
AGGAGCTGCCGTTTTATCAATATGTCAAAGAGGATATTGCAATGGTGCTTAACGGGGCGAAGGAAAAGCTCTTGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCGGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATCGAGCATCAGGACGATTTTAGTGAACTATATCGTCGTGTGCCCGCCGTCTCCTTTAAGCGTGCCAAAGCAGTAAAAG
GGGATGAGTTCGATCCGGCGCTCCTTGATGAGGCGACAGATTTGAGAAACGGCGCGAAGAAACTGCTTGAAAAGCTCAAA
ACCGACTACTTTACGAGAAGTCCTGAACAGCACTTGAAAAGCCTAACCGAAATGAAGCCTGTCATTGAAACGCTTGTACA
GCTTGTTATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATCGATTTTTCTGATTTGGAGCATT
ACTGTTTAGCGATTTTGACAGCTCAGAATGATAAAGGTGAACGTGAGCCGAGCGAGGCTGCAAGGTTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTCGTACAGGAATCGATTCTGCAGCTCGTCACAGGCGGGCC
GGAGGAGACTGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTTTCTAAATACAAACGGTTTACAGAGAGCGGGGAAGGCACGGGGCGGAAAATCGACTTGAATAAAAATTTCCGAAGC
CGTTCAGATATATTAGACAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGTCGTATCCGGACAATGACGAGACGGAAACGGAGCTGCTGCTGATCGACAACGCAG
AAGATACGGATGCAAACGAGGAAGCGGAAGAGCTTGAAACCGTGCAGTTCGAGGCAAAAGCCATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACATCGCAATATTCAATACCGAGATATTGTGAT
TTTGCTCCGCTCCATGCCGTGGGCTCCGCAAATCATGGAAGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTCGAAGCGGTCGAAGTCGCCGTCGCGCTTTCGGTGCTGAAGGTGATCGATAATCCGTATCAGGATATA
CCGCTTGCCTCTGTGCTGCGTTCACCGATTGTCGGTGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAGGCGATGAAAGACTACCTGGCTGCCGGTGACCGGAACGATGAGCTTTATCAAAAGCTGCATACGT
TTTACGGACATCTGCAAAAATGGCGTGTCTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGACTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTTTATGACAGGGC
GCGCCAATATGAATCAACGGCATTTCGCGGCTTGTTTCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGTACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATTCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCAGCGGGTCTCGGCCGGAATTTTAACATGATGGATTTAAATAAATCGTACCTGCTGGACAAGGA
GCTCGGATTCGGCACGAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACGCTGCCTCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCGTGCAAGAATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAAACTGATTGGCTCCTTCCGGA
ATTTGACCGCTATCAGGCGAAAACATATTTAGATTTCATCGGACCGGCTCTTGCCAGACATAGAGACTTGGAGGCTTTGG
CTGGAAACGGTACACCAGTACACGCTGATATCTCAGGCCATCCAGCTCGTTTTGCCGTTCAAATGATTCATTCCTATGAT
TTGCTTGATGATGATGTGGAAGAAAGAATGGAAGAAAAAAGTGAACGTCTGGAAGCGATCCGCCGAGGGGAACCAGTTCC
CGGCTCGTTTGCGTTTGATGAAAAAGCCCGCGAGCAGCTGAACTGGACCTACCCGCATCAAGAGGTGACACAAATTCGGA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCACCTGTAAAACCGGCT
GACGGAAGCATTCTGTACAGACGTCCCGCTTTTATGATGAAAAAAGGCCTGACAGCGGCAGAGAAAGGTACTGCCATGCA
TACGGTGATGCAGCATATCCCGTTGTCACATGTGCCGTCGATAGAAGAAGCTGAGCAGACGGTTCAAAGGCTTTATGAAA
AAGAGCTGCTTACTGAAGAACAAAAAGACGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGGGGA
CAGCTGATCGGTGCGAAATGGAAGGACCGGGAAATACCTTTCAGCTTGGCGCTTCCGGCTAAGGAGATCTATCCTGATGC
AGAGGAGGCGGATGAGCCGCTTTTAGTGCAGGGGATCATTGACTGTCTCTATGAAACTGAGGACGGATTATATTTATTGG
ATTATAAGTCGGACCGGATTGAGGGCAAATTTCAACATGGATTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAT
ACCCAGATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTCACATTATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1225)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-882)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	97.65	100	0.977