Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS09_RS20990 Genome accession   NZ_CP121848
Coordinates   4412520..4413659 (-) Length   379 a.a.
NCBI ID   WP_201912255.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 14430     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4407520..4418659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS09_RS20965 - 4408149..4408268 (+) 120 WP_139439872.1 oligopeptide transporter ATP-binding component -
  QBS09_RS20970 oppF 4408269..4409264 (+) 996 WP_005337666.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -
  QBS09_RS20975 - 4409567..4410511 (+) 945 WP_005353134.1 TerC family protein -
  QBS09_RS20980 alr 4410577..4411803 (-) 1227 WP_201912257.1 alanine racemase -
  QBS09_RS20985 - 4411977..4412426 (+) 450 WP_149152581.1 isoprenylcysteine carboxylmethyltransferase family protein -
  QBS09_RS20990 pilU 4412520..4413659 (-) 1140 WP_201912255.1 type IVa pilus ATPase TapW Machinery gene
  QBS09_RS20995 - 4413718..4414074 (-) 357 WP_005337656.1 DMT family protein -
  QBS09_RS21000 - 4414187..4414918 (+) 732 WP_043825634.1 transporter substrate-binding domain-containing protein -
  QBS09_RS21005 btuC 4415017..4416024 (+) 1008 WP_201912252.1 vitamin B12 ABC transporter permease BtuC -
  QBS09_RS21010 - 4416011..4416796 (+) 786 WP_201912251.1 ATP-binding cassette domain-containing protein -
  QBS09_RS21015 - 4417275..4418339 (-) 1065 WP_201912243.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42297.35 Da        Isoelectric Point: 6.4290

>NTDB_id=814730 QBS09_RS20990 WP_201912255.1 4412520..4413659(-) (pilU) [Aeromonas veronii strain ANYA 14430]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKQQFEKDLEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTDIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=814730 QBS09_RS20990 WP_201912255.1 4412520..4413659(-) (pilU) [Aeromonas veronii strain ANYA 14430]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAGTTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAGCCGGGGGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGAAGGATCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTGGCCCGCAACATCAAGACCGACATCCCCCGCTTTGA
AGATCTCAAACTCCCGCCTGTGCTGCTCGACACCATCATGGAGAAACGCGGTCTGGTACTGTTTGTCGGCGGTACCGGCT
CGGGCAAATCCACTTCGCTGGCGGCGCTTATCGACCATCGTAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAT
CCGGTAGAGTTCGTTCATCGTCACCGCAAGAGCATCATCAACCAGCGGGAGGTCGGCGTGGATACCCGCAGCTTCCACGC
AGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTCATTCTTATCGGCGAGATCCGCGATCGCGAGACCATGGAACATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCCCAGCTGCTTAACGATCTGGGCAACAACCTCAAGGCTTTTGTCTCCCAACG
GCTGGTGAAAACCACCGATGGCGGCCGCCGGGCCGCAGTGGAGATCATGCTCGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGCGGCCTCAAAGAGGTGATGGAGAAGTCCAAAGCCCTCGGCATGGTTACCTTCGACAGTGCCCTG
TTCGATCTGGTAGTGGAAGGGGTAATTGAGGAAGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGGCAGATTGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGACGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.211

100

0.544
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Pseudomonas aeruginosa PAK

41.279

90.765

0.375
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

87.863

0.367
  pilT Vibrio cholerae strain A1552

41.742

87.863

0.367
  pilT Acinetobacter baumannii D1279779

41.617

88.127

0.367
  pilT Acinetobacter baumannii strain A118

41.617

88.127

0.367
  pilT Acinetobacter nosocomialis M2

41.617

88.127

0.367