Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS09_RS15925 Genome accession   NZ_CP121848
Coordinates   3343900..3345009 (+) Length   369 a.a.
NCBI ID   WP_129504783.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 14430     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3338900..3350009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS09_RS15895 - 3339801..3340220 (-) 420 WP_201914209.1 DUF4426 domain-containing protein -
  QBS09_RS15900 yggU 3340264..3340566 (-) 303 WP_021230942.1 DUF167 family protein YggU -
  QBS09_RS15905 - 3340566..3341117 (-) 552 WP_005340343.1 YggT family protein -
  QBS09_RS15910 proC 3341129..3341953 (-) 825 WP_042053316.1 pyrroline-5-carboxylate reductase -
  QBS09_RS15915 - 3342096..3342797 (-) 702 WP_139461353.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS09_RS15920 pilT 3342837..3343871 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS09_RS15925 pilU 3343900..3345009 (+) 1110 WP_129504783.1 type IVa pilus ATPase TapU Machinery gene
  QBS09_RS15930 yaaA 3345057..3345830 (+) 774 WP_005340358.1 peroxide stress protein YaaA -
  QBS09_RS15935 - 3346025..3346450 (+) 426 WP_005340360.1 hypothetical protein -
  QBS09_RS15940 - 3346811..3347302 (-) 492 WP_201914207.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS09_RS15945 arfB 3347335..3347751 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS09_RS15950 glnK 3347953..3348291 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS09_RS15955 - 3348550..3349557 (+) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41075.13 Da        Isoelectric Point: 6.6380

>NTDB_id=814726 QBS09_RS15925 WP_129504783.1 3343900..3345009(+) (pilU) [Aeromonas veronii strain ANYA 14430]
MNLDDLLSQLVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814726 QBS09_RS15925 WP_129504783.1 3343900..3345009(+) (pilU) [Aeromonas veronii strain ANYA 14430]
ATGAATCTGGATGATCTGCTGAGCCAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAAGCGCTCGACAAGAAGGGGGCTCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATTTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGCGCATTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCTACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTTCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAATTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTTGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TTCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACTGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

55.858

99.458

0.556
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36