Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS12_RS05090 Genome accession   NZ_CP121841
Coordinates   1078522..1079631 (+) Length   369 a.a.
NCBI ID   WP_279464766.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18196     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1073522..1084631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS12_RS05060 - 1074423..1074842 (-) 420 WP_279464286.1 DUF4426 domain-containing protein -
  QBS12_RS05065 yggU 1074886..1075188 (-) 303 WP_005340341.1 DUF167 family protein YggU -
  QBS12_RS05070 - 1075188..1075739 (-) 552 WP_279464287.1 YggT family protein -
  QBS12_RS05075 proC 1075751..1076575 (-) 825 WP_279464288.1 pyrroline-5-carboxylate reductase -
  QBS12_RS05080 - 1076718..1077419 (-) 702 WP_279464289.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS12_RS05085 pilT 1077459..1078493 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS12_RS05090 pilU 1078522..1079631 (+) 1110 WP_279464766.1 type IVa pilus ATPase TapU Machinery gene
  QBS12_RS05095 yaaA 1079679..1080452 (+) 774 WP_279464290.1 peroxide stress protein YaaA -
  QBS12_RS05100 - 1080647..1081072 (+) 426 WP_005340360.1 hypothetical protein -
  QBS12_RS05105 - 1081433..1081924 (-) 492 WP_279464291.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS12_RS05110 arfB 1081957..1082373 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS12_RS05115 glnK 1082575..1082913 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS12_RS05120 - 1083172..1084179 (+) 1008 WP_005357960.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41062.09 Da        Isoelectric Point: 6.3716

>NTDB_id=814581 QBS12_RS05090 WP_279464766.1 1078522..1079631(+) (pilU) [Aeromonas veronii strain ANYA 18196]
MNLDELLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLENVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814581 QBS12_RS05090 WP_279464766.1 1078522..1079631(+) (pilU) [Aeromonas veronii strain ANYA 18196]
ATGAATCTGGATGAGCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTCAATGGCCATCTGGTGTCGCTGGGGGGTGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGTTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCTGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGACTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTAGAAGAT
CCGGTGGAGTTCGTTCATCAGCATGGTCGTAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGTGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTGCATCTGGTGCCGCAAGACAAGCACCGCCAATTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATTGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAGGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCCCATGCCGACTCGGCCAACGACCTGCGGCT
GCTGATCAAGCTCTCCGGTCGCGAGCAGCTGGGGTCCGGCACACTGGAAAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.528

91.87

0.363