Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QBS12_RS05085 Genome accession   NZ_CP121841
Coordinates   1077459..1078493 (+) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18196     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072459..1083493
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS12_RS05055 - 1073125..1074321 (+) 1197 WP_005348874.1 NupC/NupG family nucleoside CNT transporter -
  QBS12_RS05060 - 1074423..1074842 (-) 420 WP_279464286.1 DUF4426 domain-containing protein -
  QBS12_RS05065 yggU 1074886..1075188 (-) 303 WP_005340341.1 DUF167 family protein YggU -
  QBS12_RS05070 - 1075188..1075739 (-) 552 WP_279464287.1 YggT family protein -
  QBS12_RS05075 proC 1075751..1076575 (-) 825 WP_279464288.1 pyrroline-5-carboxylate reductase -
  QBS12_RS05080 - 1076718..1077419 (-) 702 WP_279464289.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS12_RS05085 pilT 1077459..1078493 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS12_RS05090 pilU 1078522..1079631 (+) 1110 WP_279464766.1 type IVa pilus ATPase TapU Machinery gene
  QBS12_RS05095 yaaA 1079679..1080452 (+) 774 WP_279464290.1 peroxide stress protein YaaA -
  QBS12_RS05100 - 1080647..1081072 (+) 426 WP_005340360.1 hypothetical protein -
  QBS12_RS05105 - 1081433..1081924 (-) 492 WP_279464291.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS12_RS05110 arfB 1081957..1082373 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS12_RS05115 glnK 1082575..1082913 (+) 339 WP_005341406.1 P-II family nitrogen regulator -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=814580 QBS12_RS05085 WP_005340354.1 1077459..1078493(+) (pilT) [Aeromonas veronii strain ANYA 18196]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=814580 QBS12_RS05085 WP_005340354.1 1077459..1078493(+) (pilT) [Aeromonas veronii strain ANYA 18196]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCTGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
AGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTACTGGTGACTGGCCCGACCGGTT
CCGGTAAGTCGACCACCCTGGCTGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATTCTCACCATCGAAGAT
CCCATCGAATTCGTCCACGAGAACAAACGCTGTCTGGTGAACCAGCGGGAAGTACACCGCGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTTTGG
CCATGACCGCCGCCGAAACTGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCGCGGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGTGGCCGGGTGGCGGCTCACGAGATCATGATGGGCATTCCTGCGGTGCGTAACCTCATTC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCCGTCGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407