Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BADSM9389_RS02335 Genome accession   NZ_AP023184
Coordinates   489374..490807 (-) Length   477 a.a.
NCBI ID   WP_172893112.1    Uniprot ID   -
Organism   Buttiauxella agrestis strain DSM 9389     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484374..495807
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BADSM9389_RS02305 (BADSM9389_04640) elbB 485933..486586 (+) 654 WP_172893109.1 isoprenoid biosynthesis glyoxalase ElbB -
  BADSM9389_RS02310 (BADSM9389_04650) mtgA 486583..487311 (+) 729 WP_172893110.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  BADSM9389_RS02315 (BADSM9389_04660) npr 487313..487585 (-) 273 WP_034498868.1 PTS phosphocarrier protein NPr -
  BADSM9389_RS02320 (BADSM9389_04670) rapZ 487582..488436 (-) 855 WP_034498867.1 RNase adapter RapZ -
  BADSM9389_RS02325 (BADSM9389_04680) ptsN 488490..488981 (-) 492 WP_172893111.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BADSM9389_RS02330 (BADSM9389_04690) hpf 489064..489351 (-) 288 WP_034498864.1 ribosome hibernation promoting factor -
  BADSM9389_RS02335 (BADSM9389_04700) rpoN/rpoN1 489374..490807 (-) 1434 WP_172893112.1 RNA polymerase factor sigma-54 Machinery gene
  BADSM9389_RS02340 (BADSM9389_04710) lptB 490856..491581 (-) 726 WP_034498860.1 LPS export ABC transporter ATP-binding protein -
  BADSM9389_RS02345 (BADSM9389_04720) lptA 491588..492151 (-) 564 WP_034498858.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  BADSM9389_RS02350 (BADSM9389_04730) lptC 492120..492695 (-) 576 WP_034498856.1 LPS export ABC transporter periplasmic protein LptC -
  BADSM9389_RS02355 (BADSM9389_04740) kdsC 492692..493258 (-) 567 WP_172893113.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  BADSM9389_RS02360 (BADSM9389_04750) kdsD 493275..494261 (-) 987 WP_115627151.1 arabinose-5-phosphate isomerase KdsD -
  BADSM9389_RS02365 (BADSM9389_04760) - 494277..495254 (-) 978 WP_172893114.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54026.14 Da        Isoelectric Point: 4.4152

>NTDB_id=81457 BADSM9389_RS02335 WP_172893112.1 489374..490807(-) (rpoN/rpoN1) [Buttiauxella agrestis strain DSM 9389]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEIETQEVRENEALDTREALEQKEMPD
ELPLDTSWDEIYTAGTPSGTGTDYIDDELPVYQGETTQSLQDYLMWQVDLTPFSDTDTAIATSIVDAVDEMGYLTVSLDE
ILESMGDEEITLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFARETPWLTEARVIVSEHLDLLANHDFRSLMRVTR
LKEEVLKEAMCLIQSLDPRPGQSIQTSEPEYVIPDVLVKKTQGRWTVELNADSIPRLKINQQYAAMGTNARNDSDSQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQGFFEFGAEHMKPMVLADIAQVVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=81457 BADSM9389_RS02335 WP_172893112.1 489374..490807(-) (rpoN/rpoN1) [Buttiauxella agrestis strain DSM 9389]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTTGCTATGACGCCACAGTTACAGCAGGCAATTCGTCTGTTGCA
GCTGTCGACGTTAGAATTACAGCAGGAACTTCAGCAAGCACTGGAAAGTAACCCACTGCTTGAGCAAACCGATCTGCATG
AAGAAATTGAAACTCAGGAAGTTCGTGAAAATGAAGCGTTAGATACTCGTGAGGCGCTCGAACAAAAAGAGATGCCCGAC
GAGTTACCGTTGGATACCAGCTGGGACGAAATTTACACCGCAGGTACACCATCGGGAACCGGAACTGACTATATTGATGA
CGAGTTACCGGTTTATCAGGGAGAAACTACCCAGTCACTCCAGGATTACCTGATGTGGCAGGTCGATCTCACCCCGTTTT
CTGATACCGACACCGCTATTGCAACGTCTATTGTTGATGCCGTTGACGAAATGGGCTATCTGACCGTTTCGCTGGATGAA
ATTCTCGAAAGCATGGGCGATGAAGAAATCACCCTCGACGAAGTGGAAGCGGTGCTCAAACGCGTGCAACGCTTCGATCC
TGTCGGCGTCGCCGCACGCGACCTTCGTGATTGCCTCCTGATCCAGCTTTCACAATTCGCGCGGGAAACACCCTGGTTAA
CCGAAGCTCGCGTGATTGTTAGCGAACATCTCGACTTGCTCGCCAACCATGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAAGAAGAAGTACTGAAAGAGGCAATGTGCCTGATTCAGTCGCTTGATCCGCGTCCGGGCCAGTCCATTCAGACCAG
CGAACCAGAGTATGTCATCCCTGATGTGTTGGTCAAAAAAACTCAGGGCCGCTGGACGGTAGAACTCAACGCCGACAGCA
TCCCACGTCTAAAAATAAATCAACAGTACGCTGCTATGGGCACCAATGCCCGCAATGACAGCGACTCACAATTTATTCGC
AGTAACCTGCAAGAAGCGAAGTGGCTGATCAAAAGCCTTGAAAGCCGCAATGATACCTTGCTGCGGGTTAGCCGCTGCAT
CGTTGAACAGCAGCAAGGCTTTTTTGAGTTCGGTGCAGAGCATATGAAACCGATGGTTCTTGCTGATATCGCCCAAGTGG
TCGAAATGCACGAATCCACCATTTCCCGCGTGACCACGCAAAAGTATTTGCACAGTCCGCGAGGCATTTTTGAATTGAAG
TATTTCTTCTCAAGTCATGTCAATACCGAGGGCGGCGGTGAAGCCTCCTCCACGGCTATTCGGGCTTTGGTGAAGAAATT
AATTGCTGCGGAAAACCCTGCGAAACCGCTGAGTGATAGCAAGTTAACGACTATGCTATCGGATCAGGGGATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATACCACCGTCAAACCAGCGTAAACAACTGGTGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.136

100

0.486

Multiple sequence alignment