Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS02_RS11670 Genome accession   NZ_CP121835
Coordinates   2476570..2477679 (+) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain ANYA 18462     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2471570..2482679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS02_RS11640 - 2472471..2472890 (-) 420 WP_279503438.1 DUF4426 domain-containing protein -
  QBS02_RS11645 yggU 2472934..2473236 (-) 303 WP_201958626.1 DUF167 family protein YggU -
  QBS02_RS11650 - 2473236..2473787 (-) 552 WP_005340343.1 YggT family protein -
  QBS02_RS11655 proC 2473799..2474623 (-) 825 WP_279503439.1 pyrroline-5-carboxylate reductase -
  QBS02_RS11660 - 2474766..2475467 (-) 702 WP_193860697.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS02_RS11665 pilT 2475507..2476541 (+) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS02_RS11670 pilU 2476570..2477679 (+) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  QBS02_RS11675 yaaA 2477727..2478500 (+) 774 WP_005340358.1 peroxide stress protein YaaA -
  QBS02_RS11680 - 2478695..2479120 (+) 426 WP_005340360.1 hypothetical protein -
  QBS02_RS11685 - 2479480..2479971 (-) 492 WP_279503443.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS02_RS11690 arfB 2479997..2480413 (-) 417 WP_021230947.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS02_RS11695 glnK 2480615..2480953 (+) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS02_RS11700 - 2481212..2482219 (+) 1008 WP_005357960.1 Fe(3+) ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=814501 QBS02_RS11670 WP_005340356.1 2476570..2477679(+) (pilU) [Aeromonas veronii strain ANYA 18462]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=814501 QBS02_RS11670 WP_005340356.1 2476570..2477679(+) (pilU) [Aeromonas veronii strain ANYA 18462]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTAAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAAGCGCTCGACAAGAAGGGGGCACTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGTTATATCCGCACCAAGGAGGCCAACTACGCGATTTATCGCGAGGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGACTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCATTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCTCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACTGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGTACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36