Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QBS23_RS18525 Genome accession   NZ_CP121833
Coordinates   3941330..3942769 (+) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain ANYA 18644     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3936330..3947769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS23_RS18495 mlaF 3936907..3937707 (-) 801 WP_279500662.1 phospholipid ABC transporter ATP-binding protein MlaF -
  QBS23_RS18500 - 3937915..3938910 (+) 996 WP_279500664.1 KpsF/GutQ family sugar-phosphate isomerase -
  QBS23_RS18505 kdsC 3938910..3939464 (+) 555 WP_101530526.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QBS23_RS18510 lptC 3939461..3940021 (+) 561 WP_005336338.1 LPS export ABC transporter periplasmic protein LptC -
  QBS23_RS18515 lptA 3940008..3940535 (+) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  QBS23_RS18520 lptB 3940539..3941264 (+) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  QBS23_RS18525 rpoN/rpoN1 3941330..3942769 (+) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  QBS23_RS18530 hpf 3942790..3943077 (+) 288 WP_005336323.1 ribosome hibernation promoting factor -
  QBS23_RS18535 ptsN 3943080..3943526 (+) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QBS23_RS18540 rapZ 3943572..3944438 (+) 867 WP_151286518.1 RNase adapter RapZ -
  QBS23_RS18545 npr 3944459..3944731 (+) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  QBS23_RS18550 - 3944749..3946653 (-) 1905 WP_201947186.1 PTS sugar transporter subunit IIA -
  QBS23_RS18555 - 3946761..3947501 (-) 741 WP_279500667.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=814476 QBS23_RS18525 WP_005336331.1 3941330..3942769(+) (rpoN/rpoN1) [Aeromonas veronii strain ANYA 18644]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=814476 QBS23_RS18525 WP_005336331.1 3941330..3942769(+) (rpoN/rpoN1) [Aeromonas veronii strain ANYA 18644]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGCTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCTCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCTCAGGAGCAGATGCCG
GACGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCTGCCGGCACCGCCCAAAGCGCCGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGTGAAACTACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACTCCTTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCCGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTCACCAGCGATGACGTAGAGGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGCATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGCACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGTCCCGGTAACGGCATCAT
CCAGAGCGACTCCCAGTATGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCCCCCCGTATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCCCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAATGACATCGCCG
AAGCGGTGGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCCCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491