Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QBS22_RS06485 Genome accession   NZ_CP121801
Coordinates   1361588..1363027 (+) Length   479 a.a.
NCBI ID   WP_126880648.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain BOND 19077     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1356588..1368027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS22_RS06455 mlaF 1357165..1357965 (-) 801 WP_005305979.1 phospholipid ABC transporter ATP-binding protein MlaF -
  QBS22_RS06460 - 1358173..1359168 (+) 996 WP_279485204.1 KpsF/GutQ family sugar-phosphate isomerase -
  QBS22_RS06465 kdsC 1359168..1359722 (+) 555 WP_017766397.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QBS22_RS06470 lptC 1359719..1360279 (+) 561 WP_005305970.1 LPS export ABC transporter periplasmic protein LptC -
  QBS22_RS06475 lptA 1360266..1360793 (+) 528 WP_010635928.1 lipopolysaccharide transport periplasmic protein LptA -
  QBS22_RS06480 lptB 1360797..1361522 (+) 726 WP_005305964.1 LPS export ABC transporter ATP-binding protein -
  QBS22_RS06485 rpoN/rpoN1 1361588..1363027 (+) 1440 WP_126880648.1 RNA polymerase factor sigma-54 Machinery gene
  QBS22_RS06490 hpf 1363049..1363336 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  QBS22_RS06495 ptsN 1363339..1363785 (+) 447 WP_005305951.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QBS22_RS06500 rapZ 1363828..1364694 (+) 867 WP_005305946.1 RNase adapter RapZ -
  QBS22_RS06505 npr 1364715..1364987 (+) 273 WP_005305943.1 PTS phosphocarrier protein NPr -
  QBS22_RS06510 - 1365003..1366907 (-) 1905 WP_279485205.1 HTH domain-containing protein -
  QBS22_RS06515 - 1367012..1367752 (-) 741 WP_279485206.1 KDGP aldolase family protein -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53862.81 Da        Isoelectric Point: 4.3584

>NTDB_id=814333 QBS22_RS06485 WP_126880648.1 1361588..1363027(+) (rpoN/rpoN1) [Aeromonas dhakensis strain BOND 19077]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPVDQVDVSQLDSSDAMAQDQMP
DELPVDTTWEEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVILEHMDLLGNRDYRTLARK
TKLRENDLKEVLDLIQQLEPRPGNGVIQSESQYVIPDVSVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=814333 QBS22_RS06485 WP_126880648.1 1361588..1363027(+) (rpoN/rpoN1) [Aeromonas dhakensis strain BOND 19077]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAAAACGACG
TTCAGGAGACCAGCCCGGTCGACCAGGTGGACGTCAGCCAGCTCGATTCCAGCGATGCCATGGCCCAGGACCAGATGCCG
GACGAACTGCCGGTGGATACCACCTGGGAAGAGGTCTACTCGGCCGGCACCGCCCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCTTGACCCCGTTCA
GCGACGTGGATGCCGCCATCGCCCTCGCCATCATAGATGCCATCGACGAATCCGGTTACCTGACGGTGTCGCTTGAGGAT
ATTCAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGATGAAGTGGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGCTCGGTGCAGGAGTGCCTGCTGATCCAGCTGGGCCAGTACTCCCCTGACTTGCCCT
GGCTCAACGAAGCCAGGGAGGTGATCCTCGAGCACATGGATCTGCTCGGCAACCGCGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTGAGAGAGAACGACCTCAAGGAGGTGCTGGATCTCATTCAGCAGCTGGAGCCGCGGCCCGGTAACGGCGTCAT
CCAGAGCGAATCCCAGTACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGCAACAAGTGGGTGGTCGAGCTCAACCCTG
ACTCGGCCCCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCAGACAGGCCCGCACCAGTCAGGATACCCAGTTC
ATCCGCTCCCACCTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTCTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCGCGGGTGACTACCCAGAAGTACATGCACACGCCGCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.866

100

0.486