Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QBJ73_RS04240 Genome accession   NZ_CP121776
Coordinates   882985..883785 (-) Length   266 a.a.
NCBI ID   WP_062031315.1    Uniprot ID   A0AAJ6LDX5
Organism   Acinetobacter johnsonii strain AYTCM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 877985..888785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBJ73_RS04220 (QBJ73_04225) - 879066..879764 (-) 699 WP_148825483.1 tetratricopeptide repeat protein -
  QBJ73_RS04225 (QBJ73_04230) hisS 879792..881087 (-) 1296 WP_308469542.1 histidine--tRNA ligase -
  QBJ73_RS04230 (QBJ73_04235) ispG 881084..882199 (-) 1116 WP_004697677.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QBJ73_RS04235 (QBJ73_04240) - 882218..882994 (-) 777 WP_062031311.1 helix-turn-helix domain-containing protein -
  QBJ73_RS04240 (QBJ73_04245) pilF 882985..883785 (-) 801 WP_062031315.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QBJ73_RS04245 (QBJ73_04250) rlmN 883802..885037 (-) 1236 WP_004697671.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  QBJ73_RS04250 (QBJ73_04255) ndk 885161..885592 (-) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  QBJ73_RS04255 (QBJ73_04260) iscX 885703..885903 (-) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  QBJ73_RS04260 (QBJ73_04265) - 885994..886545 (-) 552 WP_005401706.1 phosphatase PAP2 family protein -
  QBJ73_RS04265 (QBJ73_04270) - 886738..888018 (-) 1281 WP_308469543.1 glycosyltransferase family 1 protein -
  QBJ73_RS04270 (QBJ73_04275) - 888159..888575 (-) 417 WP_004983505.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30076.05 Da        Isoelectric Point: 9.1263

>NTDB_id=814155 QBJ73_RS04240 WP_062031315.1 882985..883785(-) (pilF) [Acinetobacter johnsonii strain AYTCM]
MKKPVSKLYLALTLGVSVWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYIQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARANHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=814155 QBJ73_RS04240 WP_062031315.1 882985..883785(-) (pilF) [Acinetobacter johnsonii strain AYTCM]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGTGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAATTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTATTG
TTACAGCGTGAAGGCAGTCCGCAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATATACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCTGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAATCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_19)

(89-147)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

68.284

100

0.688
  pilF Acinetobacter baylyi ADP1

64.286

100

0.643