Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QBK97_RS02520 Genome accession   NZ_CP121768
Coordinates   480026..480607 (-) Length   193 a.a.
NCBI ID   WP_035496524.1    Uniprot ID   -
Organism   Glaesserella parasuis strain d76     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 475026..485607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBK97_RS02480 (QBK97_02480) - 475369..476007 (+) 639 WP_035496518.1 nitroreductase family protein -
  QBK97_RS02485 (QBK97_02485) - 476096..476629 (+) 534 WP_010786060.1 TIGR00645 family protein -
  QBK97_RS02490 (QBK97_02490) - 476716..478014 (-) 1299 WP_005710399.1 FAD-binding oxidoreductase -
  QBK97_RS02510 (QBK97_02510) greB 478542..479030 (-) 489 WP_035496521.1 transcription elongation factor GreB -
  QBK97_RS02515 (QBK97_02515) - 479030..479965 (-) 936 WP_005710401.1 KpsF/GutQ family sugar-phosphate isomerase -
  QBK97_RS02520 (QBK97_02520) pilF 480026..480607 (-) 582 WP_035496524.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QBK97_RS02525 (QBK97_02525) - 480707..481867 (-) 1161 WP_010786053.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  QBK97_RS02530 (QBK97_02530) - 482068..482376 (-) 309 WP_005710405.1 helix-turn-helix domain-containing protein -
  QBK97_RS02535 (QBK97_02535) - 482380..482718 (-) 339 WP_062923967.1 toxin -
  QBK97_RS02560 (QBK97_02560) gltX 483557..484996 (+) 1440 WP_035496906.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 22323.24 Da        Isoelectric Point: 8.0872

>NTDB_id=814072 QBK97_RS02520 WP_035496524.1 480026..480607(-) (pilF) [Glaesserella parasuis strain d76]
MTFAKFFRNLTACSVALWLVACANQTTQVDFNRAEAVKARINLALAYLEQSDFPKAKENIDKALEHDVKDYLPHSVLAYY
YQQTGDNQKAEEAYQQALKLSKTQSKNNQVRPDVLNNYGTFLCKQKQFDKAYQQFETALTSQEAYYNQADTLENIALCAN
MKKDIEKQKTALSQLEKIDKSRAERLYNFMEIR

Nucleotide


Download         Length: 582 bp        

>NTDB_id=814072 QBK97_RS02520 WP_035496524.1 480026..480607(-) (pilF) [Glaesserella parasuis strain d76]
ATGACCTTTGCAAAATTTTTCCGTAATTTGACCGCTTGTAGCGTGGCATTATGGCTTGTTGCTTGTGCGAACCAAACTAC
TCAGGTGGATTTTAACCGCGCGGAAGCCGTCAAAGCTCGCATTAATTTGGCCCTTGCTTACCTTGAACAATCTGATTTTC
CAAAAGCGAAAGAGAACATTGACAAAGCGCTTGAACACGATGTAAAAGACTACCTACCGCACTCTGTGCTTGCCTATTAT
TACCAACAAACTGGCGATAACCAAAAAGCAGAAGAAGCCTACCAGCAAGCCTTGAAGTTAAGTAAAACACAGAGCAAAAA
CAATCAAGTTCGCCCTGATGTATTAAATAACTACGGTACCTTTCTTTGCAAACAAAAGCAGTTCGACAAAGCTTATCAGC
AATTTGAAACGGCATTAACCAGTCAAGAAGCCTATTATAATCAAGCAGATACACTAGAAAATATCGCCTTATGTGCCAAT
ATGAAGAAGGATATTGAGAAGCAAAAAACAGCACTATCTCAACTTGAGAAAATAGATAAATCAAGAGCAGAACGACTATA
TAATTTCATGGAGATTAGATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_2)

(74-102)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

98.964

100

0.99
  pilF/pilF2 Haemophilus influenzae Rd KW20

44.693

92.746

0.415