Detailed information NTG0814063

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: QAY87_RS31915
• Genome accession: NZ_CP121766
• Coordinates: 6796546..6797694 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 22112
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6791546..6802694

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QAY87_RS31890 (QAY87_31875)	-	6792697..6792993 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
QAY87_RS31895 (QAY87_31880)	-	6792990..6793583 (-)	594	WP_003084544.1	YggT family protein	-
QAY87_RS31900 (QAY87_31885)	proC	6793594..6794415 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
QAY87_RS31905 (QAY87_31890)	-	6794427..6795119 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
QAY87_RS31910 (QAY87_31895)	pilT	6795334..6796368 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
QAY87_RS31915 (QAY87_31900)	pilU	6796546..6797694 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
QAY87_RS31920 (QAY87_31905)	czcD	6797702..6798601 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
QAY87_RS31925 (QAY87_31910)	-	6798755..6799159 (+)	405	WP_003084560.1	NINE protein	-
QAY87_RS31930 (QAY87_31915)	-	6799402..6800775 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
QAY87_RS31935 (QAY87_31920)	-	6800772..6801956 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=814063 QAY87_RS31915 WP_003110016.1 6796546..6797694(+) (pilU) [Pseudomonas aeruginosa strain 22112]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=814063 QAY87_RS31915 WP_003110016.1 6796546..6797694(+) (pilU) [Pseudomonas aeruginosa strain 22112]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382