Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QA712_RS06610 Genome accession   NZ_CP121539
Coordinates   1214385..1215746 (-) Length   453 a.a.
NCBI ID   WP_003130563.1    Uniprot ID   -
Organism   Lactococcus lactis strain MA5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1209385..1220746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QA712_RS06575 (QA712_06580) - 1209932..1210426 (+) 495 WP_195935875.1 GNAT family N-acetyltransferase -
  QA712_RS06580 (QA712_06585) - 1210517..1210840 (-) 324 WP_029344508.1 DUF1048 domain-containing protein -
  QA712_RS06585 (QA712_06590) - 1210827..1211174 (-) 348 WP_058207950.1 DUF1048 domain-containing protein -
  QA712_RS06590 (QA712_06595) - 1211161..1211523 (-) 363 WP_003129889.1 PadR family transcriptional regulator -
  QA712_RS06595 (QA712_06600) - 1211644..1212273 (-) 630 WP_332529347.1 pyridoxamine 5'-phosphate oxidase family protein -
  QA712_RS06600 (QA712_06605) - 1212338..1213423 (-) 1086 WP_021723074.1 PIN/TRAM domain-containing protein -
  QA712_RS06605 (QA712_06610) - 1213655..1214341 (+) 687 WP_012898598.1 YoaK family protein -
  QA712_RS06610 (QA712_06615) radA 1214385..1215746 (-) 1362 WP_003130563.1 DNA repair protein RadA Machinery gene
  QA712_RS06620 (QA712_06625) rplQ 1216387..1216767 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  QA712_RS06625 (QA712_06630) - 1216786..1217724 (-) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  QA712_RS06630 (QA712_06635) rpsK 1217772..1218155 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  QA712_RS06635 (QA712_06640) rpsM 1218176..1218541 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  QA712_RS06640 (QA712_06645) rpmJ 1218559..1218675 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  QA712_RS06645 (QA712_06650) infA 1218729..1218947 (-) 219 WP_003130554.1 translation initiation factor IF-1 -
  QA712_RS06650 (QA712_06655) - 1219248..1219895 (-) 648 WP_010906298.1 adenylate kinase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49893.28 Da        Isoelectric Point: 5.1748

>NTDB_id=812864 QA712_RS06610 WP_003130563.1 1214385..1215746(-) (radA) [Lactococcus lactis strain MA5]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=812864 QA712_RS06610 WP_003130563.1 1214385..1215746(-) (radA) [Lactococcus lactis strain MA5]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTGTCAATGACTGGCGAACGTT
CAAAACCGTTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACTGACTTAGATGAATTTAATCGTGTA
TTAGGAGGAGGTGTTGTTCCTGGTTCATTAGTTCTGATTGGGGGAGACCCTGGGATAGGTAAATCAACCTTACTTTTACA
AGTTTCTACCCAATTGGCTTCTAGAGGTCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CCGAACGTTTAGGAGATATTGACCGAGATTTCTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTTTTAATTATTGATTCCATTCAAACAATTATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACCTTTATTGTTGGCCATG
TGACTAAAGAAGGACAGCTGGCCGGGCCAAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGGGAGCGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
CCATGGCTTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGCTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCCCTTGAGGGAACACGGCCCATATTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTCGAGAAGAGAACGGGGCTTTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCAGGGGGAGTAAAATTGGATGAGCCGGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGACGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACAGGTGAAATTCGC
CGGGTGACCCGAATTGAACAAAGATTGAATGAAGCTGCAAAACTAGGGTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.236

100

0.812
  radA Streptococcus pneumoniae D39

81.236

100

0.812
  radA Streptococcus pneumoniae R6

81.236

100

0.812
  radA Streptococcus pneumoniae TIGR4

81.236

100

0.812
  radA Streptococcus mitis SK321

81.236

100

0.812
  radA Streptococcus mitis NCTC 12261

81.015

100

0.81
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62