Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MY015_RS02000 Genome accession   NZ_AP026907
Coordinates   424377..424844 (-) Length   155 a.a.
NCBI ID   WP_000340171.1    Uniprot ID   -
Organism   Escherichia coli strain MY732     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 419377..429844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MY015_RS01975 (MY016_03870) rpsG 419518..419988 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  MY015_RS01980 (MY016_03880) fusA 420085..422199 (+) 2115 WP_000124700.1 elongation factor G -
  MY015_RS01985 (MY016_03890) tuf 422270..423454 (+) 1185 WP_000031783.1 elongation factor Tu -
  MY015_RS01990 (MY016_03900) bfd 423637..423831 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  MY015_RS01995 (MY016_03910) bfr 423904..424380 (+) 477 WP_000675515.1 bacterioferritin -
  MY015_RS02000 (MY016_03920) pilD 424377..424844 (-) 468 WP_000340171.1 prepilin peptidase Machinery gene
  MY015_RS02005 (MY016_03930) rpsJ 425223..425534 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  MY015_RS02010 (MY016_03940) rplC 425567..426196 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  MY015_RS02015 (MY016_03950) rplD 426207..426812 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  MY015_RS02020 (MY016_03960) rplW 426809..427111 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  MY015_RS02025 (MY016_03970) rplB 427129..427950 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  MY015_RS02030 (MY016_03980) rpsS 427967..428245 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  MY015_RS02035 (MY016_03990) rplV 428260..428592 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  MY015_RS02040 (MY016_04000) rpsC 428610..429311 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  MY015_RS02045 (MY016_04010) rplP 429324..429734 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16938.08 Da        Isoelectric Point: 8.1296

>NTDB_id=81246 MY015_RS02000 WP_000340171.1 424377..424844(-) (pilD) [Escherichia coli strain MY732]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=81246 MY015_RS02000 WP_000340171.1 424377..424844(-) (pilD) [Escherichia coli strain MY732]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361

Multiple sequence alignment