Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RTE58_RS02620 Genome accession   NZ_CP135959
Coordinates   541614..543047 (-) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain MS1266     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 536614..548047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RTE58_RS02585 (RTE58_02585) elbB 537306..537959 (+) 654 WP_001302019.1 isoprenoid biosynthesis glyoxalase ElbB -
  RTE58_RS02590 (RTE58_02590) mtgA 537956..538684 (+) 729 WP_000047098.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  RTE58_RS02595 (RTE58_02595) yrbL 538681..539313 (-) 633 WP_137509908.1 PhoP regulatory network protein YrbL -
  RTE58_RS02600 (RTE58_02600) npr 539526..539798 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  RTE58_RS02605 (RTE58_02605) rapZ 539795..540649 (-) 855 WP_000243741.1 RNase adapter RapZ -
  RTE58_RS02610 (RTE58_02610) ptsN 540695..541186 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RTE58_RS02615 (RTE58_02615) hpf 541304..541591 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  RTE58_RS02620 (RTE58_02620) rpoN/rpoN1 541614..543047 (-) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  RTE58_RS02625 (RTE58_02625) lptB 543095..543820 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  RTE58_RS02630 (RTE58_02630) lptA 543827..544384 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  RTE58_RS02635 (RTE58_02635) lptC 544353..544928 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  RTE58_RS02640 (RTE58_02640) kdsC 544925..545491 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  RTE58_RS02645 (RTE58_02645) kdsD 545512..546498 (-) 987 WP_085455557.1 arabinose-5-phosphate isomerase KdsD -
  RTE58_RS02650 (RTE58_02650) yrbG 546512..547489 (-) 978 WP_094258990.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=812458 RTE58_RS02620 WP_000809051.1 541614..543047(-) (rpoN/rpoN1) [Escherichia coli strain MS1266]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=812458 RTE58_RS02620 WP_000809051.1 541614..543047(-) (rpoN/rpoN1) [Escherichia coli strain MS1266]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCAAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCATGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAATATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTTTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465