Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RTE58_RS01960 Genome accession   NZ_CP135959
Coordinates   422125..422802 (-) Length   225 a.a.
NCBI ID   WP_061336009.1    Uniprot ID   -
Organism   Escherichia coli strain MS1266     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417125..427802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RTE58_RS01945 (RTE58_01945) chiA 418483..421185 (+) 2703 WP_096939278.1 bifunctional chitinase/lysozyme -
  RTE58_RS01950 (RTE58_01950) bfd 421354..421548 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RTE58_RS01955 (RTE58_01955) bfr 421620..422096 (+) 477 WP_000675504.1 bacterioferritin -
  RTE58_RS01960 (RTE58_01960) pilD 422125..422802 (-) 678 WP_061336009.1 prepilin peptidase GspO Machinery gene
  RTE58_RS01965 (RTE58_01965) gspM 422802..423263 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  RTE58_RS01970 (RTE58_01970) gspL 423260..424423 (-) 1164 WP_001335091.1 type II secretion system protein GspL -
  RTE58_RS01975 (RTE58_01975) gspK 424438..425421 (-) 984 WP_317732043.1 type II secretion system minor pseudopilin GspK -
  RTE58_RS01980 (RTE58_01980) gspJ 425414..426001 (-) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  RTE58_RS01985 (RTE58_01985) gspI 425994..426371 (-) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  RTE58_RS01990 (RTE58_01990) gspH 426368..426877 (-) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  RTE58_RS01995 (RTE58_01995) gspG 426885..427322 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24824.67 Da        Isoelectric Point: 7.6364

>NTDB_id=812455 RTE58_RS01960 WP_061336009.1 422125..422802(-) (pilD) [Escherichia coli strain MS1266]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVTAAIACALAPFTPIVTGAL
FLYLCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTVTTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=812455 RTE58_RS01960 WP_061336009.1 422125..422802(-) (pilD) [Escherichia coli strain MS1266]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTACTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTATGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTGTAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.576

100

0.382