Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Thiofri_RS14205 Genome accession   NZ_CP121471
Coordinates   3087516..3088622 (-) Length   368 a.a.
NCBI ID   WP_143741765.1    Uniprot ID   -
Organism   Thiorhodovibrio frisius strain 970     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3082516..3093622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Thiofri_RS14185 (Thiofri_02906) - 3082595..3083557 (-) 963 WP_009147081.1 GGDEF domain-containing protein -
  Thiofri_RS14190 (Thiofri_02907) - 3083735..3084619 (-) 885 WP_009147080.1 lytic transglycosylase domain-containing protein -
  Thiofri_RS14195 (Thiofri_02908) - 3084990..3086501 (-) 1512 WP_009151691.1 transposase -
  Thiofri_RS14200 (Thiofri_02909) - 3086623..3087492 (+) 870 WP_323705279.1 hypothetical protein -
  Thiofri_RS14205 (Thiofri_02910) pilU 3087516..3088622 (-) 1107 WP_143741765.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Thiofri_RS14210 (Thiofri_02911) - 3088733..3089842 (-) 1110 WP_009147077.1 osmoprotectant NAGGN system M42 family peptidase -
  Thiofri_RS14215 (Thiofri_02912) ngg 3089839..3091593 (-) 1755 WP_009147076.1 N-acetylglutaminylglutamine synthetase -
  Thiofri_RS14220 (Thiofri_02913) - 3091734..3093509 (-) 1776 WP_009147075.1 N-acetylglutaminylglutamine amidotransferase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41171.16 Da        Isoelectric Point: 5.5902

>NTDB_id=812296 Thiofri_RS14205 WP_143741765.1 3087516..3088622(-) (pilU) [Thiorhodovibrio frisius strain 970]
MDFKQLLATMVAQQASDLFITAHLPPSLKIEGVIKPVENAAPLAGADVRTLVFGVMSDKQRAEFEESHECQFALVVEGVG
RFRVSAFMERDRPGMVLRRIESRVPTVEELNLPPMLVELSNASRGLLLLVGGNSSGKSTSLAALIGHRNRHTNGHIITIE
DPIEFLHEHDGCIVTQREVGVDTDAVEVALHHSLRQAPDVVMIGEIRSRRALEEVISFVEAGHLVLSSIHAQNYRQALDR
MVNLFPEDSRRQILSELGLNLVGIVGQMLVPCKDDGGVLPAFEILVNTPLVAETIRRDDFDSLKEIMLRNFDQGMSTFDN
SLFQLYRDGRVSMDEALNHAESRNDVRLMMKMHDREVGVRERQAERQK

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=812296 Thiofri_RS14205 WP_143741765.1 3087516..3088622(-) (pilU) [Thiorhodovibrio frisius strain 970]
TTGGACTTTAAGCAATTACTTGCAACCATGGTCGCGCAACAGGCATCCGACCTATTCATTACAGCGCATCTGCCGCCGAG
CTTGAAGATCGAGGGTGTCATCAAGCCGGTTGAGAATGCCGCGCCCTTGGCAGGGGCGGATGTCCGCACCCTGGTGTTTG
GGGTGATGAGCGATAAACAGCGCGCTGAGTTCGAGGAAAGCCACGAATGCCAGTTTGCGCTTGTAGTAGAAGGCGTCGGG
CGCTTTCGGGTCAGCGCCTTCATGGAGCGCGACCGTCCGGGTATGGTGCTGCGACGCATTGAGTCGCGCGTGCCGACGGT
CGAGGAACTCAACCTGCCCCCGATGCTGGTGGAGCTTTCCAACGCCAGTCGCGGACTGTTGCTGCTGGTTGGCGGCAATT
CGTCCGGCAAATCAACTTCGTTGGCGGCGCTGATTGGGCACCGCAACCGCCATACGAATGGGCACATCATCACCATCGAA
GACCCGATTGAATTTCTCCATGAACATGATGGATGTATTGTTACTCAGAGGGAGGTTGGTGTAGATACCGATGCGGTTGA
GGTCGCCCTGCATCATTCGCTGCGTCAGGCACCGGATGTGGTCATGATTGGCGAGATTCGCAGCCGCCGGGCGTTGGAAG
AGGTGATCTCTTTCGTCGAGGCCGGGCATCTGGTGCTCTCGAGCATCCATGCGCAAAATTACCGGCAGGCGCTTGATCGC
ATGGTTAATCTCTTTCCCGAGGATTCCCGCCGGCAGATATTGTCGGAACTGGGGCTGAATCTGGTGGGGATCGTCGGGCA
AATGCTGGTGCCTTGCAAGGACGATGGCGGTGTGTTGCCAGCGTTCGAGATTCTGGTGAACACCCCGCTGGTGGCCGAGA
CCATCCGGCGCGATGATTTCGACTCCCTGAAGGAGATCATGCTCAGGAATTTCGATCAGGGTATGTCAACCTTCGATAAC
AGCTTGTTCCAGCTCTATCGTGATGGACGCGTGTCGATGGATGAAGCCCTGAACCATGCCGAATCGCGCAACGATGTGCG
GCTGATGATGAAAATGCACGACCGCGAAGTTGGCGTGCGAGAGCGTCAGGCCGAGCGTCAGAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.409

95.652

0.511
  pilU Acinetobacter baylyi ADP1

48.925

100

0.495
  pilU Vibrio cholerae strain A1552

49.573

95.38

0.473