Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   P9J59_RS02495 Genome accession   NZ_CP121365
Coordinates   516992..517792 (+) Length   266 a.a.
NCBI ID   WP_005135836.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain Ab_8_4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511992..522792
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P9J59_RS02470 (P9J59_02470) - 512902..514200 (+) 1299 WP_086238243.1 glycosyltransferase family 1 protein -
  P9J59_RS02475 (P9J59_02475) - 514225..514776 (+) 552 WP_000669563.1 phosphatase PAP2 family protein -
  P9J59_RS02480 (P9J59_02480) iscX 514876..515076 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  P9J59_RS02485 (P9J59_02485) ndk 515190..515621 (+) 432 WP_264425890.1 nucleoside-diphosphate kinase -
  P9J59_RS02490 (P9J59_02490) rlmN 515744..516976 (+) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  P9J59_RS02495 (P9J59_02495) pilF 516992..517792 (+) 801 WP_005135836.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  P9J59_RS02500 (P9J59_02500) - 517783..518574 (+) 792 WP_000405163.1 RodZ domain-containing protein -
  P9J59_RS02505 (P9J59_02505) ispG 518596..519711 (+) 1116 WP_264425889.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  P9J59_RS02510 (P9J59_02510) hisS 519708..521000 (+) 1293 WP_000095251.1 histidine--tRNA ligase -
  P9J59_RS02515 (P9J59_02515) - 521029..521727 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30120.52 Da        Isoelectric Point: 9.2307

>NTDB_id=811947 P9J59_RS02495 WP_005135836.1 516992..517792(+) (pilF) [Acinetobacter baumannii strain Ab_8_4]
MSTPKLKIMLCMGVAVALLASGCQTLQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=811947 P9J59_RS02495 WP_005135836.1 516992..517792(+) (pilF) [Acinetobacter baumannii strain Ab_8_4]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTTGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCCCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCGGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726