Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   P3S51_RS10525 Genome accession   NZ_CP121303
Coordinates   2134379..2135182 (-) Length   267 a.a.
NCBI ID   WP_407061092.1    Uniprot ID   -
Organism   Acinetobacter sp. ANC 7201     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2129379..2140182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3S51_RS10505 (P3S51_10505) - 2130418..2131119 (-) 702 WP_311917861.1 tetratricopeptide repeat protein -
  P3S51_RS10510 (P3S51_10510) hisS 2131150..2132445 (-) 1296 WP_311917862.1 histidine--tRNA ligase -
  P3S51_RS10515 (P3S51_10515) ispG 2132449..2133570 (-) 1122 WP_154772104.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  P3S51_RS10520 (P3S51_10520) - 2133594..2134388 (-) 795 WP_154772103.1 helix-turn-helix domain-containing protein -
  P3S51_RS10525 (P3S51_10525) pilF 2134379..2135182 (-) 804 WP_407061092.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  P3S51_RS10530 (P3S51_10530) rlmN 2135201..2136433 (-) 1233 WP_154772102.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  P3S51_RS10535 (P3S51_10535) ndk 2136558..2136989 (-) 432 WP_120401584.1 nucleoside-diphosphate kinase -
  P3S51_RS10540 (P3S51_10540) iscX 2137097..2137297 (-) 201 WP_154772101.1 Fe-S cluster assembly protein IscX -
  P3S51_RS10545 (P3S51_10545) - 2137439..2137990 (-) 552 WP_154772100.1 phosphatase PAP2 family protein -
  P3S51_RS10550 (P3S51_10550) - 2138023..2139297 (-) 1275 WP_311917863.1 glycosyltransferase family 1 protein -
  P3S51_RS10555 (P3S51_10555) - 2139382..2140002 (-) 621 WP_311917864.1 DUF2971 domain-containing protein -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30274.38 Da        Isoelectric Point: 9.6286

>NTDB_id=811751 P3S51_RS10525 WP_407061092.1 2134379..2135182(-) (pilF) [Acinetobacter sp. ANC 7201]
MRKPNLKIFISSFVVGATFLVVGCQTVSDRKVDPEKAVQARTQLAAEYIRTGDFDAAKRELDQAMQVNPRNALVNMMMGV
LLQREGSQLNMEKADRYFKAAISADPKNAQARNNYGTYLYQAGRYNEALTHLEIAGATLGYDQRYTAFESAGRIYLKLND
VNKAENSFNQALKISRNSYISMVELAEIYYLRQDIPAATRLYENSVSLAGQNNQNARALWVGIRLARANGNPMQTQVLVN
QLRALYPDSPEYQRYLQLQYSTEVVWK

Nucleotide


Download         Length: 804 bp        

>NTDB_id=811751 P3S51_RS10525 WP_407061092.1 2134379..2135182(-) (pilF) [Acinetobacter sp. ANC 7201]
TTGCGTAAACCTAATCTGAAAATATTCATCTCATCATTTGTTGTGGGTGCTACTTTTTTAGTTGTGGGTTGTCAGACAGT
ATCTGATCGCAAGGTTGATCCTGAAAAAGCAGTTCAAGCAAGAACGCAGTTGGCTGCTGAATATATTCGAACTGGCGACT
TTGATGCAGCAAAACGTGAATTAGACCAAGCTATGCAGGTTAATCCACGTAATGCACTTGTAAATATGATGATGGGTGTG
TTGTTGCAACGTGAAGGTAGTCAGCTCAATATGGAAAAAGCTGATCGCTACTTTAAAGCAGCCATTTCAGCAGATCCTAA
AAATGCGCAAGCTCGTAACAACTATGGTACTTATTTGTATCAAGCTGGTCGTTATAATGAAGCATTAACGCACTTAGAGA
TTGCAGGTGCAACATTAGGTTATGATCAGCGTTATACTGCATTTGAAAGTGCGGGACGTATTTATTTAAAGCTAAATGAT
GTTAATAAGGCTGAAAATAGCTTTAATCAAGCTTTAAAAATTAGCCGTAATTCATATATCTCAATGGTCGAATTGGCTGA
GATATATTATTTAAGACAAGATATTCCTGCTGCGACTAGACTTTATGAAAATTCTGTCAGTTTAGCTGGGCAAAATAATC
AAAATGCACGTGCTTTGTGGGTGGGTATTCGACTTGCACGCGCAAATGGTAATCCTATGCAAACACAGGTTCTTGTGAAT
CAATTACGTGCTTTGTATCCGGATAGTCCAGAATACCAACGCTATTTGCAATTACAGTACAGTACTGAGGTCGTATGGAA
GTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(98-129)

Tetratricopeptide repeat (TPR_8)

(151-177)

Tetratricopeptide repeat (TPR_8)

(39-70)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

65.918

100

0.659
  pilF Acinetobacter baylyi ADP1

63.218

97.753

0.618