Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RS163_RS01945 Genome accession   NZ_CP135688
Coordinates   423021..423698 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain TUM2820     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418021..428698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS163_RS01930 (RS163_01930) chiA 419388..422081 (+) 2694 WP_072663100.1 bifunctional chitinase/lysozyme -
  RS163_RS01935 (RS163_01935) bfd 422250..422444 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RS163_RS01940 (RS163_01940) bfr 422516..422992 (+) 477 WP_000675504.1 bacterioferritin -
  RS163_RS01945 (RS163_01945) pilD 423021..423698 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  RS163_RS01950 (RS163_01950) gspM 423698..424159 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  RS163_RS01955 (RS163_01955) gspL 424156..425319 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  RS163_RS01960 (RS163_01960) gspK 425334..426317 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RS163_RS01965 (RS163_01965) gspJ 426310..426897 (-) 588 WP_196107197.1 type II secretion system minor pseudopilin GspJ -
  RS163_RS01970 (RS163_01970) gspI 426890..427267 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RS163_RS01975 (RS163_01975) gspH 427264..427773 (-) 510 WP_076486713.1 type II secretion system minor pseudopilin GspH -
  RS163_RS01980 (RS163_01980) gspG 427781..428218 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=811701 RS163_RS01945 WP_000178161.1 423021..423698(-) (pilD) [Escherichia coli strain TUM2820]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=811701 RS163_RS01945 WP_000178161.1 423021..423698(-) (pilD) [Escherichia coli strain TUM2820]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378