Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RS165_RS03615 Genome accession   NZ_CP135680
Coordinates   724552..724992 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain TUM3755     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 719552..729992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS165_RS03590 (RS165_03590) coaE 720042..720662 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  RS165_RS03595 (RS165_03595) - 720687..720731 (+) 45 WP_120795372.1 protein YacM -
  RS165_RS03600 (RS165_03600) guaC 720887..721930 (+) 1044 WP_001217338.1 GMP reductase -
  RS165_RS03605 (RS165_03605) hofC 721965..723167 (-) 1203 WP_000157266.1 protein transport protein HofC -
  RS165_RS03610 (RS165_03610) pilB 723157..724542 (-) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  RS165_RS03615 (RS165_03615) pilA 724552..724992 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  RS165_RS03620 (RS165_03620) nadC 725195..726088 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RS165_RS03625 (RS165_03625) ampD 726176..726727 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RS165_RS03630 (RS165_03630) ampE 726724..727578 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  RS165_RS03635 (RS165_03635) aroP 727621..728994 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=811535 RS165_RS03615 WP_000360904.1 724552..724992(-) (pilA) [Escherichia coli strain TUM3755]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=811535 RS165_RS03615 WP_000360904.1 724552..724992(-) (pilA) [Escherichia coli strain TUM3755]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363