Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P8R49_RS00580 Genome accession   NZ_CP121266
Coordinates   105631..106998 (+) Length   455 a.a.
NCBI ID   WP_284603695.1    Uniprot ID   -
Organism   Bacillus subtilis strain 107105     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100631..111998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8R49_RS00560 (P8R49_00560) ctsR 100984..101448 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  P8R49_RS00565 (P8R49_00565) mcsA 101462..102019 (+) 558 WP_015252993.1 protein-arginine kinase activator protein McsA -
  P8R49_RS00570 (P8R49_00570) mcsB 102019..103110 (+) 1092 WP_003235007.1 protein arginine kinase -
  P8R49_RS00575 (P8R49_00575) clpC 103107..105539 (+) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  P8R49_RS00580 (P8R49_00580) radA 105631..106998 (+) 1368 WP_284603695.1 DNA repair protein RadA Machinery gene
  P8R49_RS00585 (P8R49_00585) disA 107002..108084 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  P8R49_RS00590 (P8R49_00590) yacL 108200..109300 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  P8R49_RS00595 (P8R49_00595) ispD 109315..110013 (+) 699 WP_014478616.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  P8R49_RS00600 (P8R49_00600) ispF 110006..110482 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49137.64 Da        Isoelectric Point: 8.4735

>NTDB_id=811479 P8R49_RS00580 WP_284603695.1 105631..106998(+) (radA) [Bacillus subtilis strain 107105]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSFGNP
RRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIVISIASSFRDTPPNPADCFIGEVGLTGEV
RRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=811479 P8R49_RS00580 WP_284603695.1 105631..106998(+) (radA) [Bacillus subtilis strain 107105]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTGGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACTGCTGAGCTGATGAAAATCGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAGGCTCGAGTA
TCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTTGGCAACCCA
AGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGGACTGCTGCT
GCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCGATTGTGATTA
GTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACCGGAGAAGTC
CGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGCTTTAAACGCATGATCATACCCGCGGCAAA
TCTGGACGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTACTTCATTAG
GAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(362-427)

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.127

100

0.998
  radA Streptococcus pneumoniae Rx1

62.914

99.56

0.626
  radA Streptococcus pneumoniae D39

62.914

99.56

0.626
  radA Streptococcus pneumoniae R6

62.914

99.56

0.626
  radA Streptococcus pneumoniae TIGR4

62.914

99.56

0.626
  radA Streptococcus mitis NCTC 12261

62.914

99.56

0.626
  radA Streptococcus mitis SK321

62.693

99.56

0.624