Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RS169_RS15575 Genome accession   NZ_CP135656
Coordinates   3223887..3224564 (-) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain TUM11356     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3218887..3229564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS169_RS15560 (RS169_15560) chiA 3220245..3222947 (+) 2703 WP_000773180.1 bifunctional chitinase/lysozyme -
  RS169_RS15565 (RS169_15565) bfd 3223116..3223310 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RS169_RS15570 (RS169_15570) bfr 3223382..3223858 (+) 477 WP_000675504.1 bacterioferritin -
  RS169_RS15575 (RS169_15575) pilD 3223887..3224564 (-) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  RS169_RS15580 (RS169_15580) gspM 3224564..3225025 (-) 462 WP_001296465.1 type II secretion system protein GspM -
  RS169_RS15585 (RS169_15585) gspL 3225022..3226185 (-) 1164 WP_001296464.1 type II secretion system protein GspL -
  RS169_RS15590 (RS169_15590) gspK 3226200..3227183 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  RS169_RS15595 (RS169_15595) gspJ 3227176..3227763 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  RS169_RS15600 (RS169_15600) gspI 3227756..3228133 (-) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  RS169_RS15605 (RS169_15605) gspH 3228130..3228639 (-) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  RS169_RS15610 (RS169_15610) gspG 3228647..3229084 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=811229 RS169_RS15575 WP_000178170.1 3223887..3224564(-) (pilD) [Escherichia coli strain TUM11356]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=811229 RS169_RS15575 WP_000178170.1 3223887..3224564(-) (pilD) [Escherichia coli strain TUM11356]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382