Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RS171_RS17300 Genome accession   NZ_CP135649
Coordinates   3479457..3480938 (-) Length   493 a.a.
NCBI ID   WP_032279022.1    Uniprot ID   -
Organism   Escherichia coli strain TUM14664     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3474457..3485938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS171_RS17270 (RS171_17270) gspK 3475370..3476353 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RS171_RS17275 (RS171_17275) gspJ 3476346..3476933 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  RS171_RS17280 (RS171_17280) gspI 3476926..3477303 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RS171_RS17285 (RS171_17285) gspH 3477300..3477809 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  RS171_RS17290 (RS171_17290) gspG 3477817..3478254 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  RS171_RS17295 (RS171_17295) gspF 3478264..3479460 (-) 1197 WP_074576916.1 type II secretion system inner membrane protein GspF -
  RS171_RS17300 (RS171_17300) pilB 3479457..3480938 (-) 1482 WP_032279022.1 type II secretion system ATPase GspE Machinery gene
  RS171_RS17305 (RS171_17305) gspD 3480948..3482900 (-) 1953 WP_109963383.1 type II secretion system secretin GspD -
  RS171_RS17310 (RS171_17310) gspC 3482884..3483441 (-) 558 Protein_3382 type II secretion system protein GspC -
  RS171_RS17315 (RS171_17315) - 3483509..3484489 (+) 981 WP_000019450.1 IS5-like element ISKpn26 family transposase -
  RS171_RS17320 (RS171_17320) - 3484471..3485622 (+) 1152 WP_315747905.1 peptidoglycan-binding domain-containing protein -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54638.62 Da        Isoelectric Point: 6.2412

>NTDB_id=811065 RS171_RS17300 WP_032279022.1 3479457..3480938(-) (pilB) [Escherichia coli strain TUM14664]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINVILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=811065 RS171_RS17300 WP_032279022.1 3479457..3480938(-) (pilB) [Escherichia coli strain TUM14664]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGTGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCTGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAAGGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(115-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.682

88.641

0.414
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Acinetobacter baumannii D1279779

45.578

89.452

0.408
  pilB Legionella pneumophila strain ERS1305867

41.372

97.566

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

46.353

86.207

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

41.176

96.552

0.398
  pilB Acinetobacter baylyi ADP1

48.338

79.31

0.383
  pilF Thermus thermophilus HB27

45.316

80.122

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.178

79.919

0.361