Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RS171_RS17255 Genome accession   NZ_CP135649
Coordinates   3473057..3473734 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain TUM14664     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3468057..3478734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS171_RS17240 (RS171_17240) chiA 3469424..3472117 (+) 2694 WP_032279025.1 bifunctional chitinase/lysozyme -
  RS171_RS17245 (RS171_17245) bfd 3472286..3472480 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RS171_RS17250 (RS171_17250) bfr 3472552..3473028 (+) 477 WP_000675504.1 bacterioferritin -
  RS171_RS17255 (RS171_17255) pilD 3473057..3473734 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  RS171_RS17260 (RS171_17260) gspM 3473734..3474195 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  RS171_RS17265 (RS171_17265) gspL 3474192..3475355 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  RS171_RS17270 (RS171_17270) gspK 3475370..3476353 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  RS171_RS17275 (RS171_17275) gspJ 3476346..3476933 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  RS171_RS17280 (RS171_17280) gspI 3476926..3477303 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  RS171_RS17285 (RS171_17285) gspH 3477300..3477809 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  RS171_RS17290 (RS171_17290) gspG 3477817..3478254 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=811064 RS171_RS17255 WP_000178161.1 3473057..3473734(-) (pilD) [Escherichia coli strain TUM14664]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=811064 RS171_RS17255 WP_000178161.1 3473057..3473734(-) (pilD) [Escherichia coli strain TUM14664]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378