Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P8F79_RS13380 Genome accession   NZ_CP121172
Coordinates   2689666..2691036 (-) Length   456 a.a.
NCBI ID   WP_025904845.1    Uniprot ID   A0A2T4RU06
Organism   Mammaliicoccus sciuri strain PC04     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2684666..2696036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8F79_RS13355 (P8F79_13350) cysE 2685059..2685700 (-) 642 WP_025904850.1 serine O-acetyltransferase -
  P8F79_RS13360 (P8F79_13355) gltX 2685955..2687409 (-) 1455 WP_048541341.1 glutamate--tRNA ligase -
  P8F79_RS13365 (P8F79_13360) ispF 2687425..2687904 (-) 480 WP_048541339.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  P8F79_RS13370 (P8F79_13365) ispD 2687897..2688583 (-) 687 WP_048541338.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  P8F79_RS13375 (P8F79_13370) - 2688585..2689643 (-) 1059 WP_048541336.1 PIN/TRAM domain-containing protein -
  P8F79_RS13380 (P8F79_13375) radA 2689666..2691036 (-) 1371 WP_025904845.1 DNA repair protein RadA Machinery gene
  P8F79_RS13385 (P8F79_13380) - 2691192..2693654 (-) 2463 WP_025904844.1 ATP-dependent Clp protease ATP-binding subunit -
  P8F79_RS13390 (P8F79_13385) - 2693671..2694678 (-) 1008 WP_048541334.1 protein arginine kinase -
  P8F79_RS13395 (P8F79_13390) - 2694679..2695215 (-) 537 WP_048541333.1 UvrB/UvrC motif-containing protein -
  P8F79_RS13400 (P8F79_13395) - 2695230..2695691 (-) 462 WP_025904841.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50477.24 Da        Isoelectric Point: 7.7103

>NTDB_id=810852 P8F79_RS13380 WP_025904845.1 2689666..2691036(-) (radA) [Mammaliicoccus sciuri strain PC04]
MAKKKVIFECLACGYQSPKWMGKCPNCGAWNQMEESIEHKETKGPKNTFKSDVPAQKIEKLKDVKEEDTPRIKTKINEFN
RVLGGGVVEGSLVLIGGDPGIGKSTLLLQICAALSQQKNVLYVSGEESVRQTKLRAERLVEDSGELNVYAETNLEIIHSM
VQKEKPDVLVIDSIQTVFHPEVTSAPGSVSQVRECTQNLMHIAKQMNIATFIVGHVTKDGQIAGPRLLEHMVDTVLYFEG
DTHHAYRILRAVKNRFGSTNEMGIFEMKQTGLNEVKNPSEMFLEERTKHVSGSTIVATMEGTRPILVEVQALVTPTTFHN
PRRMATGIDHNRLSLLMAVLEKKENFLLQQQDAYIKIAGGVKLSEPAVDLSIVIAIASSFQDKPTKGDDCFVGEVGLTGE
VRRVARIDQRVQEAAKLGFKRIIIPKNNIKGLEFPEGTEVIGVSTTREALKIAFNA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=810852 P8F79_RS13380 WP_025904845.1 2689666..2691036(-) (radA) [Mammaliicoccus sciuri strain PC04]
ATGGCCAAAAAGAAAGTAATATTTGAGTGTTTAGCCTGCGGATATCAATCTCCAAAATGGATGGGGAAATGCCCTAATTG
TGGTGCATGGAACCAAATGGAAGAATCAATAGAACATAAAGAAACTAAAGGTCCTAAAAATACATTCAAGTCAGATGTAC
CCGCACAAAAAATAGAAAAATTAAAAGACGTTAAAGAAGAAGATACACCTAGAATTAAGACTAAAATCAATGAATTTAAT
CGTGTATTAGGTGGCGGTGTTGTAGAAGGTTCGCTTGTACTTATTGGTGGTGACCCTGGTATTGGTAAATCCACATTATT
ATTACAAATTTGTGCGGCTTTATCTCAACAAAAAAATGTCTTGTATGTTTCAGGTGAAGAATCTGTAAGACAAACGAAAT
TAAGAGCTGAAAGATTAGTTGAAGATTCAGGAGAATTAAATGTTTATGCTGAAACGAATTTAGAGATTATACATAGTATG
GTTCAAAAAGAAAAACCAGATGTATTAGTTATAGATTCCATTCAAACTGTCTTCCACCCTGAAGTTACTTCTGCTCCAGG
TTCTGTTTCTCAAGTTAGAGAATGTACACAAAATTTAATGCATATCGCAAAACAAATGAACATTGCTACATTTATTGTAG
GACATGTAACGAAAGATGGACAAATTGCTGGGCCACGTTTGTTAGAACATATGGTTGATACAGTATTATATTTTGAAGGC
GATACACATCATGCATACAGAATATTAAGAGCAGTTAAAAACCGTTTTGGTTCGACAAATGAAATGGGTATTTTTGAAAT
GAAGCAAACGGGTCTTAATGAAGTTAAAAATCCGTCTGAAATGTTCTTAGAAGAAAGAACGAAACATGTATCGGGTTCGA
CTATTGTGGCAACTATGGAAGGTACGAGACCTATTTTAGTTGAAGTACAAGCTTTAGTAACACCAACGACTTTTCATAAT
CCACGAAGAATGGCAACAGGTATTGATCACAATAGATTAAGCTTATTGATGGCTGTATTAGAGAAGAAAGAGAATTTCTT
ATTACAACAACAAGACGCATATATCAAAATTGCCGGTGGTGTAAAATTAAGTGAACCCGCTGTAGATTTAAGCATTGTTA
TCGCGATAGCTTCAAGTTTCCAAGATAAACCTACTAAAGGTGATGATTGTTTCGTGGGTGAGGTTGGCTTAACAGGTGAA
GTAAGAAGGGTTGCTAGAATTGATCAACGTGTACAAGAAGCGGCTAAATTAGGATTTAAACGTATTATCATACCTAAAAA
TAATATTAAAGGTTTAGAATTCCCGGAAGGTACAGAAGTTATTGGCGTATCAACAACAAGAGAAGCATTGAAAATTGCTT
TTAATGCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(349-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4RU06

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.721

100

0.66
  radA Streptococcus pneumoniae Rx1

61.894

99.561

0.616
  radA Streptococcus pneumoniae D39

61.894

99.561

0.616
  radA Streptococcus pneumoniae R6

61.894

99.561

0.616
  radA Streptococcus pneumoniae TIGR4

61.894

99.561

0.616
  radA Streptococcus mitis NCTC 12261

61.894

99.561

0.616
  radA Streptococcus mitis SK321

61.894

99.561

0.616