Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RS173_RS01960 Genome accession   NZ_CP135643
Coordinates   421531..422208 (-) Length   225 a.a.
NCBI ID   WP_000178172.1    Uniprot ID   -
Organism   Escherichia coli strain TUM17543     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 416531..427208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS173_RS01945 (RS173_01945) chiA 417892..420591 (+) 2700 Protein_385 bifunctional chitinase/lysozyme -
  RS173_RS01950 (RS173_01950) bfd 420760..420954 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  RS173_RS01955 (RS173_01955) bfr 421026..421502 (+) 477 WP_000675504.1 bacterioferritin -
  RS173_RS01960 (RS173_01960) pilD 421531..422208 (-) 678 WP_000178172.1 prepilin peptidase GspO Machinery gene
  RS173_RS01965 (RS173_01965) gspM 422208..422669 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  RS173_RS01970 (RS173_01970) gspL 422666..423829 (-) 1164 WP_001304915.1 type II secretion system protein GspL -
  RS173_RS01975 (RS173_01975) gspK 423844..424827 (-) 984 WP_001058529.1 type II secretion system minor pseudopilin GspK -
  RS173_RS01980 (RS173_01980) gspJ 424820..425407 (-) 588 WP_000610637.1 type II secretion system minor pseudopilin GspJ -
  RS173_RS01985 (RS173_01985) gspI 425400..425777 (-) 378 WP_001041742.1 type II secretion system minor pseudopilin GspI -
  RS173_RS01990 (RS173_01990) gspH 425774..426283 (-) 510 WP_001076033.1 type II secretion system minor pseudopilin GspH -
  RS173_RS01995 (RS173_01995) gspG 426291..426728 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.70 Da        Isoelectric Point: 7.6364

>NTDB_id=810844 RS173_RS01960 WP_000178172.1 421531..422208(-) (pilD) [Escherichia coli strain TUM17543]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTAPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTIPTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=810844 RS173_RS01960 WP_000178172.1 421531..422208(-) (pilD) [Escherichia coli strain TUM17543]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGCGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTACCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATACCCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382