Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P8F76_RS00185 Genome accession   NZ_CP121167
Coordinates   44166..45536 (+) Length   456 a.a.
NCBI ID   WP_002364585.1    Uniprot ID   -
Organism   Enterococcus faecalis strain PC15     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 39166..50536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8F76_RS00160 (P8F76_00160) - 39656..40123 (-) 468 WP_002364587.1 VOC family protein -
  P8F76_RS00165 (P8F76_00165) - 40188..41135 (+) 948 WP_002385468.1 YafY family protein -
  P8F76_RS00170 (P8F76_00170) - 41227..42480 (-) 1254 WP_002399100.1 glutamate-5-semialdehyde dehydrogenase -
  P8F76_RS00175 (P8F76_00175) proB 42467..43282 (-) 816 WP_002389550.1 glutamate 5-kinase -
  P8F76_RS00180 (P8F76_00180) - 43602..44087 (+) 486 WP_002361472.1 dUTP diphosphatase -
  P8F76_RS00185 (P8F76_00185) radA 44166..45536 (+) 1371 WP_002364585.1 DNA repair protein RadA Machinery gene
  P8F76_RS00190 (P8F76_00190) - 45637..46782 (+) 1146 WP_002368622.1 PIN/TRAM domain-containing protein -
  P8F76_RS00195 (P8F76_00195) ispF 46801..47274 (+) 474 WP_002359233.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  P8F76_RS00200 (P8F76_00200) gltX 47327..48784 (+) 1458 WP_002381335.1 glutamate--tRNA ligase -
  P8F76_RS00205 (P8F76_00205) epsC 49054..49593 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49770.36 Da        Isoelectric Point: 8.0317

>NTDB_id=810792 P8F76_RS00185 WP_002364585.1 44166..45536(+) (radA) [Enterococcus faecalis strain PC15]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVIPKKEPRVKTELVELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMQTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWKAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=810792 P8F76_RS00185 WP_002364585.1 44166..45536(+) (radA) [Enterococcus faecalis strain PC15]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGCCGCTGTCCTAA
TTGTGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTCATACCTAAAAAAGAGCCTCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTTCCAGGTTCGTTAGTTTTAATCGGTGGCGATCCCGGAATAGGTAAGTCAACATTGCT
CTTACAAGTATCGCAACAATTAGCAGCCACAGGTGGTACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTACGAGCGGAGCGTCTAGGCACCGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATATCACGA
GCCATTGAAAAATTAGAACCAGACTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAAGTCCGAGAAACAACTGCCGAATTATTGAAACTGGCAAAAACGAATGGGATTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGGCCACGGATGTTGGAACATATGGTAGACACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACCTTTAGAATCTTACGGGCTGTCAAAAATCGGTTTGGCTCAACGAATGAGATTGGTATTTTTGA
AATGCAGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGAGCCACCGGAT
CTTCAATTGTGGTAGCAATGGAAGGTTCACGCCCCATTTTGGTGGAAATCCAAGCGTTAGTTACCCCAACGATGTTTGGC
AATGCCAAGCGAACGACCACCGGGTTGGACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGGGCAGGCCT
TTTGTTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCAGAATGTTTCATTGGTGAGATTGGTTTAACTGGT
GAAATCCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGAAAGCGCCTGAAGGGATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

75.385

99.781

0.752
  radA Streptococcus pneumoniae Rx1

75.385

99.781

0.752
  radA Streptococcus pneumoniae D39

75.385

99.781

0.752
  radA Streptococcus pneumoniae R6

75.385

99.781

0.752
  radA Streptococcus pneumoniae TIGR4

75.385

99.781

0.752
  radA Streptococcus mitis SK321

75.385

99.781

0.752
  radA Bacillus subtilis subsp. subtilis str. 168

66.297

98.904

0.656