Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P8F83_RS16045 Genome accession   NZ_CP121166
Coordinates   3121254..3122618 (-) Length   454 a.a.
NCBI ID   WP_021800128.1    Uniprot ID   U2NTH3
Organism   Clostridium intestinale strain PC17     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3116254..3127618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8F83_RS16025 (P8F83_16025) ispD 3117635..3118309 (-) 675 WP_238905908.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  P8F83_RS16030 (P8F83_16030) - 3118329..3119420 (-) 1092 WP_073022783.1 PIN/TRAM domain-containing protein -
  P8F83_RS16035 (P8F83_16035) - 3119743..3120141 (+) 399 WP_021800130.1 hypothetical protein -
  P8F83_RS16040 (P8F83_16040) disA 3120174..3121238 (-) 1065 WP_326511870.1 DNA integrity scanning diadenylate cyclase DisA -
  P8F83_RS16045 (P8F83_16045) radA 3121254..3122618 (-) 1365 WP_021800128.1 DNA repair protein RadA Machinery gene
  P8F83_RS16050 (P8F83_16050) nagB 3122817..3123545 (-) 729 WP_326511871.1 glucosamine-6-phosphate deaminase -
  P8F83_RS16055 (P8F83_16055) - 3123542..3124264 (-) 723 WP_326511872.1 GntR family transcriptional regulator -
  P8F83_RS16060 (P8F83_16060) - 3124388..3124549 (+) 162 WP_326511873.1 hypothetical protein -
  P8F83_RS16065 (P8F83_16065) - 3124690..3124917 (+) 228 WP_021800125.1 glutaredoxin family protein -
  P8F83_RS16070 (P8F83_16070) - 3124969..3126426 (-) 1458 WP_326511874.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  P8F83_RS16075 (P8F83_16075) - 3126442..3126669 (-) 228 WP_073022789.1 glutaredoxin family protein -
  P8F83_RS16080 (P8F83_16080) trxB 3126669..3127610 (-) 942 WP_326511875.1 thioredoxin-disulfide reductase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 50321.21 Da        Isoelectric Point: 7.6378

>NTDB_id=810781 P8F83_RS16045 WP_021800128.1 3121254..3122618(-) (radA) [Clostridium intestinale strain PC17]
MAKIKTMYVCQECGYESAKWLGKCPSCNSWNSFQEEVKENKNDSRNKNFLSGTISLPKNIREIQSGEKERLDTGVKELNR
VLGGGLVRGSLTLISGDPGIGKSTLLLQTAQNIASKYGKVLYVSGEESEEQIKIRGDRLGVESNNLFIVSETNLEIVESH
IKELSPIFVIVDSIQTMYKEGIASAPGSVSQVKECSNVIMRIAKHNNIPLFIVAHVTKQGELAGPRVLEHMVDTVLSFEG
ERTEEFRILRTMKNRFGTTREIGVFEMRGEGLVEVFDPSKLFLQEKDENQEGSVVIGLMEGSRPILVEIQALVTETKAVM
PRRTAVGIENSRLSLILAVLEKKLKIPFYKYDVYINVVGGLNIDGTTADLGVAVALLSSIKNKEVALPRVMLVGEVGLTG
EIRPISACERIVNEAEKMGFKNVIIPDRNKDKIEAKTLNIHGVRNLREAINKIF

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=810781 P8F83_RS16045 WP_021800128.1 3121254..3122618(-) (radA) [Clostridium intestinale strain PC17]
ATGGCTAAAATAAAAACAATGTATGTATGTCAAGAATGTGGCTACGAATCTGCAAAATGGTTAGGGAAATGCCCCTCTTG
TAATTCGTGGAATAGCTTTCAAGAAGAAGTTAAAGAAAATAAAAATGATAGTAGAAATAAAAACTTTTTATCCGGAACAA
TAAGTTTACCTAAAAATATCAGAGAAATACAATCTGGAGAAAAGGAAAGACTTGATACTGGAGTAAAAGAATTAAATAGA
GTACTTGGAGGAGGGCTAGTAAGAGGTTCATTAACCCTTATATCTGGAGATCCAGGTATCGGAAAATCAACCCTTTTACT
ACAAACAGCCCAAAATATAGCAAGTAAATATGGAAAAGTATTATATGTATCTGGTGAGGAATCAGAGGAACAAATAAAAA
TTAGAGGAGATAGATTAGGGGTAGAGTCAAACAATCTATTCATTGTTTCAGAGACAAACTTAGAGATAGTAGAATCTCAT
ATAAAAGAATTGTCTCCTATATTTGTTATAGTAGATTCTATACAAACAATGTATAAAGAAGGAATAGCTTCAGCCCCTGG
ATCAGTTTCACAGGTAAAAGAATGCTCTAACGTAATCATGAGAATAGCAAAACACAATAATATACCCTTGTTTATTGTTG
CCCATGTAACTAAGCAGGGTGAGCTTGCAGGACCAAGAGTGTTAGAACATATGGTGGATACCGTTCTTTCATTTGAAGGC
GAAAGAACGGAAGAATTCAGAATACTAAGAACCATGAAAAATCGTTTTGGAACAACTAGAGAAATTGGTGTTTTTGAGAT
GAGAGGAGAAGGGCTTGTAGAAGTATTTGACCCATCTAAGTTATTCCTTCAAGAAAAAGATGAGAATCAAGAAGGTTCGG
TGGTTATAGGACTAATGGAAGGTTCTAGACCTATACTAGTAGAAATACAAGCTTTAGTAACGGAGACTAAGGCAGTAATG
CCTAGAAGAACTGCAGTAGGTATAGAAAACTCTAGGCTTAGCTTGATACTTGCAGTTTTAGAGAAAAAGCTAAAGATACC
TTTTTATAAATACGATGTATATATTAACGTGGTTGGTGGTTTGAATATAGATGGAACAACAGCTGATCTTGGAGTTGCTG
TTGCATTATTATCTTCTATAAAAAATAAAGAGGTTGCACTGCCAAGAGTTATGCTAGTAGGGGAAGTGGGTCTAACAGGC
GAAATTAGGCCAATATCAGCATGTGAAAGAATAGTAAATGAAGCTGAAAAAATGGGATTTAAAAATGTAATAATTCCAGA
TAGAAATAAAGATAAGATTGAAGCAAAAACTTTAAATATACACGGAGTAAGAAATCTTAGAGAAGCTATAAATAAAATAT
TTTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(71-220)

Subunit ChlI of Mg-chelatase (ChlI)

(344-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U2NTH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

51.214

99.78

0.511
  radA Streptococcus pneumoniae Rx1

49.451

100

0.496
  radA Streptococcus pneumoniae D39

49.451

100

0.496
  radA Streptococcus pneumoniae R6

49.451

100

0.496
  radA Streptococcus pneumoniae TIGR4

49.451

100

0.496
  radA Streptococcus mitis NCTC 12261

49.451

100

0.496
  radA Streptococcus mitis SK321

49.231

100

0.493