Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P8F82_RS06630 Genome accession   NZ_CP121162
Coordinates   1414842..1415816 (+) Length   324 a.a.
NCBI ID   WP_107543212.1    Uniprot ID   A0A5R9B885
Organism   Staphylococcus xylosus strain PC20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1409842..1420816
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8F82_RS06595 (P8F82_06595) rpmG 1409951..1410100 (+) 150 WP_002483181.1 50S ribosomal protein L33 -
  P8F82_RS06600 (P8F82_06600) - 1410232..1410774 (+) 543 WP_042362613.1 5-formyltetrahydrofolate cyclo-ligase -
  P8F82_RS06605 (P8F82_06605) - 1410786..1412243 (+) 1458 WP_069794317.1 rhomboid family intramembrane serine protease -
  P8F82_RS06610 (P8F82_06610) - 1412227..1412427 (+) 201 WP_107542787.1 YqgQ family protein -
  P8F82_RS06615 (P8F82_06615) - 1412809..1413795 (+) 987 WP_029377187.1 glucokinase -
  P8F82_RS06620 (P8F82_06620) - 1413795..1414118 (+) 324 WP_042362616.1 MTH1187 family thiamine-binding protein -
  P8F82_RS06625 (P8F82_06625) - 1414120..1414743 (+) 624 WP_042362617.1 MBL fold metallo-hydrolase -
  P8F82_RS06630 (P8F82_06630) comGA 1414842..1415816 (+) 975 WP_107543212.1 competence type IV pilus ATPase ComGA Machinery gene
  P8F82_RS06635 (P8F82_06635) comGB 1415788..1416855 (+) 1068 WP_107543211.1 competence type IV pilus assembly protein ComGB -
  P8F82_RS06640 (P8F82_06640) comGC 1416875..1417192 (+) 318 WP_029377192.1 competence type IV pilus major pilin ComGC Machinery gene
  P8F82_RS06645 (P8F82_06645) comGD 1417170..1417616 (+) 447 WP_225526499.1 competence type IV pilus minor pilin ComGD -
  P8F82_RS06650 (P8F82_06650) - 1417603..1417902 (+) 300 WP_119610859.1 hypothetical protein -
  P8F82_RS06655 (P8F82_06655) comGF 1417886..1418317 (+) 432 WP_326001449.1 competence type IV pilus minor pilin ComGF -
  P8F82_RS06660 (P8F82_06660) - 1418482..1418997 (+) 516 WP_171031808.1 shikimate kinase -
  P8F82_RS06665 (P8F82_06665) gcvT 1419185..1420276 (+) 1092 WP_042362624.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 37165.35 Da        Isoelectric Point: 9.3719

>NTDB_id=810745 P8F82_RS06630 WP_107543212.1 1414842..1415816(+) (comGA) [Staphylococcus xylosus strain PC20]
MKLLFNSMIEKAINNDASDIHFIPADKEVMIKFRIRDDLKSIETLQLNTYLKLLTYMKYQAGLDVSKHNAAQSGRYIYKL
KQIYFLRISTLPLSLGNESCVIRIIPQYFQKHKNNQNITHLYNLMNKKQGLILLSGPTGSGKSTLMYHMLSYAKENLNLN
IITIEEPVEQLINGITQISLNEKAGINYETSFKAILRCDPDIILIGEIRDANVAKHVIQASLSGHLVLSTIHASNCKGVI
LRLLEMGITVQQLIQSITSILNQRLITNVKGERELVYEQITKEDIYHFFKNSYTLPKDFHNLSYKLNKMSKEGMICETSM
ERYL

Nucleotide


Download         Length: 975 bp        

>NTDB_id=810745 P8F82_RS06630 WP_107543212.1 1414842..1415816(+) (comGA) [Staphylococcus xylosus strain PC20]
TTGAAGCTATTGTTTAATAGTATGATTGAAAAGGCAATTAATAATGATGCATCAGATATTCATTTTATCCCCGCAGATAA
AGAAGTAATGATTAAATTTAGAATACGTGATGATTTGAAGTCTATAGAAACATTGCAATTAAATACGTATTTAAAATTAT
TAACCTATATGAAATACCAAGCTGGATTAGATGTCTCTAAACACAATGCTGCACAGAGTGGTAGATATATTTATAAATTA
AAACAAATTTACTTCCTTAGAATATCAACGCTTCCTTTATCTCTAGGAAATGAAAGCTGTGTTATTAGGATTATTCCGCA
ATATTTTCAAAAGCATAAAAATAATCAGAACATAACGCATTTATATAATCTAATGAATAAAAAACAAGGATTAATTTTAT
TGAGTGGACCTACTGGCTCTGGAAAAAGTACGTTAATGTATCACATGTTATCTTATGCTAAAGAGAACTTAAATTTAAAT
ATAATTACAATTGAAGAGCCCGTTGAACAATTGATAAATGGCATAACTCAAATATCATTAAATGAAAAGGCAGGGATAAA
TTATGAAACTTCTTTTAAAGCTATTTTACGCTGCGATCCTGATATTATCTTGATTGGAGAAATAAGAGATGCAAACGTAG
CTAAACATGTTATACAAGCCAGTTTAAGTGGTCATTTAGTACTTTCGACAATTCATGCTAGTAATTGCAAAGGTGTTATC
TTAAGACTGCTAGAAATGGGGATAACGGTTCAACAACTCATACAATCAATCACTAGCATATTAAATCAAAGGCTTATTAC
TAACGTAAAAGGTGAAAGAGAGCTTGTATATGAACAGATAACTAAAGAAGATATTTATCACTTCTTTAAAAATAGCTATA
CATTACCCAAAGATTTTCATAACTTATCTTATAAACTAAATAAAATGTCAAAAGAGGGGATGATATGTGAAACATCAATG
GAACGTTATCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5R9B885

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

62.654

100

0.627
  comGA Staphylococcus aureus N315

62.654

100

0.627