Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P8F80_RS02220 Genome accession   NZ_CP121161
Coordinates   477274..478644 (+) Length   456 a.a.
NCBI ID   WP_326516550.1    Uniprot ID   -
Organism   Ligilactobacillus murinus strain PC39     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 472274..483644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P8F80_RS02195 (P8F80_02195) - 473336..474007 (+) 672 WP_004051778.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  P8F80_RS02200 (P8F80_02200) - 474055..475389 (-) 1335 WP_004051777.1 C1 family peptidase -
  P8F80_RS02205 (P8F80_02205) rpiA 475526..476212 (-) 687 WP_004051776.1 ribose-5-phosphate isomerase RpiA -
  P8F80_RS02210 (P8F80_02210) - 476237..476524 (-) 288 WP_039936561.1 GNAT family N-acetyltransferase -
  P8F80_RS02215 (P8F80_02215) - 476661..477197 (+) 537 WP_004051774.1 dUTP pyrophosphatase -
  P8F80_RS02220 (P8F80_02220) radA 477274..478644 (+) 1371 WP_326516550.1 DNA repair protein RadA Machinery gene
  P8F80_RS02225 (P8F80_02225) - 478663..479772 (+) 1110 WP_135057617.1 PIN/TRAM domain-containing protein -
  P8F80_RS02230 (P8F80_02230) gltX 479861..481351 (+) 1491 WP_056960043.1 glutamate--tRNA ligase -
  P8F80_RS02235 (P8F80_02235) cysS 481718..483127 (+) 1410 WP_004051768.1 cysteine--tRNA ligase -
  P8F80_RS02240 (P8F80_02240) - 483129..483524 (+) 396 WP_004051767.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49250.59 Da        Isoelectric Point: 6.9502

>NTDB_id=810708 P8F80_RS02220 WP_326516550.1 477274..478644(+) (radA) [Ligilactobacillus murinus strain PC39]
MAKVKTRYVCQNCGYVSPRYLGRCPNCSEWNTLVEEVEQKSAAVKATPRVTLTGTTNAPQKIDTIKISQTPRVDTKNGEL
NRGLGGGVVPGSMVLIGGDPGIGKSTLLLQVSGSLSDQGGKLLYVSGEESANQIKMRAQRLGVSGSEFYLYPETDMGSIL
QNIEDLKPDYVIIDSVQTMQMPEVTSAVGSVAQIREVTAALMQVAKTNGITIFIVGHVTKGGAIAGPKILEHMVDTVLYF
EGDMHRTYRILRAVKNRFGSTNEIGVFEMRDGGLVEVPNPSEIFLEERLSGATGSAVVVSMEGTRPILAEIQALVTPTAF
GNAKRTTTGLDHNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAMSLVSSYRDQQTAPDDCFIGELGLT
GEIRRVTRIDQRIAEAAKLGFRRVFIPQNNLNGLELPTNIDVVGVKTISEALRHVF

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=810708 P8F80_RS02220 WP_326516550.1 477274..478644(+) (radA) [Ligilactobacillus murinus strain PC39]
ATGGCAAAAGTCAAAACTAGATATGTATGTCAAAATTGTGGCTATGTATCACCACGTTATTTGGGGCGGTGTCCTAACTG
TAGCGAATGGAACACTTTAGTTGAAGAAGTTGAACAAAAAAGTGCAGCGGTTAAAGCTACCCCACGCGTTACTTTGACAG
GAACAACGAATGCCCCACAAAAGATCGATACGATCAAGATCTCACAAACACCTAGAGTCGATACGAAAAATGGTGAATTG
AATCGGGGCTTAGGTGGGGGCGTTGTTCCTGGTTCAATGGTCTTGATCGGGGGCGATCCTGGGATCGGTAAATCAACGTT
ACTTTTACAAGTTTCGGGAAGTTTATCAGATCAAGGGGGAAAGTTGCTCTACGTTTCGGGTGAAGAAAGTGCAAACCAGA
TCAAGATGCGGGCCCAACGCTTGGGAGTCTCAGGCAGTGAATTTTACCTTTACCCTGAGACCGATATGGGCAGTATTTTA
CAAAATATCGAAGACTTAAAACCAGACTATGTCATCATTGACTCAGTTCAAACGATGCAGATGCCAGAAGTTACTTCGGC
GGTCGGAAGTGTGGCGCAGATCCGCGAGGTGACTGCTGCTTTGATGCAAGTTGCTAAGACAAATGGGATCACGATCTTTA
TCGTTGGTCATGTGACCAAAGGTGGCGCGATCGCTGGGCCCAAGATCTTAGAGCATATGGTCGATACGGTGTTGTACTTT
GAAGGTGACATGCATCGCACCTACCGGATCTTACGTGCAGTCAAAAATCGTTTTGGTTCGACTAATGAGATCGGTGTGTT
TGAGATGCGAGATGGCGGGCTAGTTGAAGTCCCTAATCCATCTGAGATCTTCTTAGAAGAGCGTTTAAGTGGTGCGACCG
GTTCGGCTGTCGTGGTCTCAATGGAAGGAACACGGCCGATCTTGGCAGAGATCCAAGCGTTAGTCACCCCAACAGCCTTT
GGCAACGCTAAGCGGACCACGACCGGACTAGATCACAATCGGGTCTCGTTGATCATGGCAGTGCTCGAAAAGCGGGCTGG
GCTACTTTTACAAAATCAAGATGCTTACTTAAAAGCCGCTGGGGGCGTTAAATTAGATGAACCAGCGATCGATCTAGCTA
TCGCGATGAGTTTGGTATCGAGTTATCGTGACCAACAGACCGCACCAGATGACTGTTTTATCGGTGAATTAGGTTTGACC
GGTGAGATCAGACGGGTGACTCGGATCGACCAAAGAATAGCTGAAGCAGCTAAATTAGGCTTTAGACGGGTCTTTATCCC
CCAAAATAATTTAAATGGTCTGGAATTGCCAACTAATATCGATGTAGTTGGCGTTAAGACGATCAGTGAAGCTTTACGGC
ATGTTTTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(84-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

68.202

100

0.682
  radA Streptococcus pneumoniae D39

68.202

100

0.682
  radA Streptococcus pneumoniae R6

68.202

100

0.682
  radA Streptococcus pneumoniae TIGR4

68.202

100

0.682
  radA Streptococcus mitis SK321

67.982

100

0.68
  radA Streptococcus mitis NCTC 12261

67.982

100

0.68
  radA Bacillus subtilis subsp. subtilis str. 168

62.693

99.342

0.623