Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACLCAB_RS02150 Genome accession   NZ_CP135244
Coordinates   461498..461965 (-) Length   155 a.a.
NCBI ID   WP_000340171.1    Uniprot ID   -
Organism   Escherichia coli strain OHT76-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 456498..466965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLCAB_RS02125 (RRJ52_02125) rpsG 456639..457109 (+) 471 WP_001138043.1 30S ribosomal protein S7 -
  ACLCAB_RS02130 (RRJ52_02130) fusA 457206..459320 (+) 2115 WP_000124700.1 elongation factor G -
  ACLCAB_RS02135 (RRJ52_02135) tuf 459391..460575 (+) 1185 WP_000031783.1 elongation factor Tu -
  ACLCAB_RS02140 (RRJ52_02140) bfd 460758..460952 (+) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  ACLCAB_RS02145 (RRJ52_02145) bfr 461025..461501 (+) 477 WP_000675515.1 bacterioferritin -
  ACLCAB_RS02150 (RRJ52_02150) pilD 461498..461965 (-) 468 WP_000340171.1 prepilin peptidase Machinery gene
  ACLCAB_RS02155 (RRJ52_02155) rpsJ 462344..462655 (+) 312 WP_001181004.1 30S ribosomal protein S10 -
  ACLCAB_RS02160 (RRJ52_02160) rplC 462688..463317 (+) 630 WP_000579833.1 50S ribosomal protein L3 -
  ACLCAB_RS02165 (RRJ52_02165) rplD 463328..463933 (+) 606 WP_000424395.1 50S ribosomal protein L4 -
  ACLCAB_RS02170 (RRJ52_02170) rplW 463930..464232 (+) 303 WP_000617544.1 50S ribosomal protein L23 -
  ACLCAB_RS02175 (RRJ52_02175) rplB 464250..465071 (+) 822 WP_000301864.1 50S ribosomal protein L2 -
  ACLCAB_RS02180 (RRJ52_02180) rpsS 465088..465366 (+) 279 WP_001138117.1 30S ribosomal protein S19 -
  ACLCAB_RS02185 (RRJ52_02185) rplV 465381..465713 (+) 333 WP_000447529.1 50S ribosomal protein L22 -
  ACLCAB_RS02190 (RRJ52_02190) rpsC 465731..466432 (+) 702 WP_000529945.1 30S ribosomal protein S3 -
  ACLCAB_RS02195 (RRJ52_02195) rplP 466445..466855 (+) 411 WP_000941212.1 50S ribosomal protein L16 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16938.08 Da        Isoelectric Point: 8.1296

>NTDB_id=810529 ACLCAB_RS02150 WP_000340171.1 461498..461965(-) (pilD) [Escherichia coli strain OHT76-1]
MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFSQVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHTWTFLPRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=810529 ACLCAB_RS02150 WP_000340171.1 461498..461965(-) (pilD) [Escherichia coli strain OHT76-1]
ATGGACGCTACCCTTCCCTTTCTGATTTTGTACGCTTGTTTATCTGTCCTACTTTTTTTATGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACCTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTCTCAGGTTTGTAATCCTGACGGTTTAGCCG
ATGCGTTATGGGGGGCGATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGTCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGTGCCTGGCATACCTGGACCTTCTTGCCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTGTTGGTCTGCTCATGAGAGGAAAAGAGTCATTAAAAAACCCGCTGC
CGTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTGTAGGTTGGGATAGTTTGCTGGCAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

44.8

80.645

0.361
  pilD Neisseria gonorrhoeae MS11

38.095

94.839

0.361