Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilH   Type   Machinery gene
Locus tag   ROT04_RS02040 Genome accession   NZ_CP135174
Coordinates   431413..431778 (+) Length   121 a.a.
NCBI ID   WP_003084587.1    Uniprot ID   A0A0H2ZKW2
Organism   Pseudomonas aeruginosa strain PARM_L1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 426413..436778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ROT04_RS02010 (ROT04_02010) pyrR 427035..427547 (-) 513 WP_003084574.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ROT04_RS02015 (ROT04_02015) ruvX 427659..428093 (-) 435 WP_003084575.1 Holliday junction resolvase RuvX -
  ROT04_RS02020 (ROT04_02020) - 428093..428662 (-) 570 WP_003084577.1 YqgE/AlgH family protein -
  ROT04_RS02025 (ROT04_02025) - 428711..429604 (-) 894 WP_003084580.1 TonB family protein -
  ROT04_RS02030 (ROT04_02030) gshB 429751..430704 (-) 954 WP_003100943.1 glutathione synthase -
  ROT04_RS02035 (ROT04_02035) pilG 430959..431366 (+) 408 WP_003084583.1 twitching motility response regulator PilG Regulator
  ROT04_RS02040 (ROT04_02040) pilH 431413..431778 (+) 366 WP_003084587.1 twitching motility response regulator PilH Machinery gene
  ROT04_RS02045 (ROT04_02045) - 431829..432365 (+) 537 WP_003084590.1 chemotaxis protein CheW -
  ROT04_RS02050 (ROT04_02050) pilJ 432450..434498 (+) 2049 WP_003084592.1 chemotaxis chemoreceptor PilJ -
  ROT04_RS02055 (ROT04_02055) pilK 434559..435434 (+) 876 WP_003100938.1 type 4 fimbrial methyltransferase PilK -

Sequence


Protein


Download         Length: 121 a.a.        Molecular weight: 13273.30 Da        Isoelectric Point: 5.1961

>NTDB_id=810325 ROT04_RS02040 WP_003084587.1 431413..431778(+) (pilH) [Pseudomonas aeruginosa strain PARM_L1]
MARILIVDDSPTEMYKLTAMLEKHGHQVLKAENGGDGVALARQEKPDVVLMDIVMPGLNGFQATRQLTKDAETSAIPVII
VTTKDQETDKVWGKRQGARDYLTKPVDEETLLKTINAVLAG

Nucleotide


Download         Length: 366 bp        

>NTDB_id=810325 ROT04_RS02040 WP_003084587.1 431413..431778(+) (pilH) [Pseudomonas aeruginosa strain PARM_L1]
ATGGCTCGTATTTTGATTGTTGATGACTCTCCGACCGAGATGTACAAGCTGACCGCCATGCTGGAAAAGCATGGTCACCA
GGTACTCAAGGCCGAGAACGGCGGCGACGGCGTCGCCCTGGCCCGCCAGGAAAAGCCCGACGTGGTCCTGATGGACATCG
TCATGCCCGGCCTCAACGGCTTCCAGGCGACCCGACAGTTGACCAAGGACGCCGAGACCAGCGCCATCCCGGTGATCATC
GTCACCACCAAGGACCAGGAGACCGACAAGGTCTGGGGCAAGCGCCAGGGCGCGCGCGACTACCTGACCAAGCCGGTGGA
CGAAGAGACCCTGCTGAAAACCATCAATGCGGTGCTGGCGGGCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0H2ZKW2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

36.975

98.347

0.364