Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RQ453_RS17255 Genome accession   NZ_CP135173
Coordinates   3686764..3688257 (-) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain CP-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3681764..3693257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RQ453_RS17220 (RQ453_17220) - 3682615..3683064 (+) 450 WP_003094369.1 hypothetical protein -
  RQ453_RS17225 (RQ453_17225) - 3683077..3683688 (+) 612 WP_003098862.1 superoxide dismutase -
  RQ453_RS17230 (RQ453_17230) - 3683707..3684639 (+) 933 WP_003120918.1 ZIP family metal transporter -
  RQ453_RS17235 (RQ453_17235) - 3684736..3685008 (-) 273 WP_023104882.1 HPr family phosphocarrier protein -
  RQ453_RS17240 (RQ453_17240) rapZ 3685038..3685898 (-) 861 WP_003094361.1 RNase adapter RapZ -
  RQ453_RS17245 (RQ453_17245) ptsN 3685900..3686364 (-) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RQ453_RS17250 (RQ453_17250) hpf 3686378..3686686 (-) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RQ453_RS17255 (RQ453_17255) rpoN/rpoN1 3686764..3688257 (-) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  RQ453_RS17260 (RQ453_17260) lptB 3688483..3689208 (-) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  RQ453_RS17265 (RQ453_17265) lptA 3689208..3689735 (-) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  RQ453_RS17270 (RQ453_17270) lptC 3689722..3690294 (-) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  RQ453_RS17275 (RQ453_17275) - 3690303..3690842 (-) 540 WP_003094350.1 KdsC family phosphatase -
  RQ453_RS17280 (RQ453_17280) kdsD 3690842..3691822 (-) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  RQ453_RS17285 (RQ453_17285) - 3692235..3693044 (+) 810 WP_031636640.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=810283 RQ453_RS17255 WP_003094357.1 3686764..3688257(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain CP-1]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=810283 RQ453_RS17255 WP_003094357.1 3686764..3688257(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain CP-1]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCCCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCTCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAACTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCTGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477