Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   HT085_RS08580 Genome accession   NZ_AP023069
Coordinates   1637903..1639135 (+) Length   410 a.a.
NCBI ID   WP_003689811.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain TUM19854     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1632903..1644135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT085_RS08565 (TUM19854C_16440) fabI 1633779..1634564 (-) 786 WP_002223367.1 enoyl-ACP reductase FabI -
  HT085_RS08570 (TUM19854C_16450) dapD 1634719..1635540 (-) 822 WP_003689804.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  HT085_RS08575 (TUM19854C_16460) pgi 1635750..1637393 (-) 1644 WP_003689806.1 glucose-6-phosphate isomerase -
  HT085_RS08580 (TUM19854C_16470) pilG 1637903..1639135 (+) 1233 WP_003689811.1 type 4 pilus assembly protein PilG Machinery gene
  HT085_RS08585 (TUM19854C_16480) pilD 1639209..1640069 (+) 861 WP_003689814.1 prepilin peptidase Machinery gene
  HT085_RS08590 (TUM19854C_16490) coaE 1640066..1640698 (+) 633 WP_003689816.1 dephospho-CoA kinase -
  HT085_RS08595 - 1640691..1640900 (+) 210 WP_003696096.1 DNA gyrase inhibitor YacG -
  HT085_RS08600 (TUM19854C_16500) pilF 1640986..1642662 (-) 1677 WP_003689817.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HT085_RS08610 (TUM19854C_16510) - 1643000..1643446 (-) 447 WP_003689818.1 DUF441 domain-containing protein -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45332.36 Da        Isoelectric Point: 10.0085

>NTDB_id=80996 HT085_RS08580 WP_003689811.1 1637903..1639135(+) (pilG) [Neisseria gonorrhoeae strain TUM19854]
MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARKKLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLS
TMIKAGLPLMQAFEIVARGHGNPSMTEMLMEIRGQVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPPLTQTVMDMSDFFVSYGWMVLIALGFAI
YGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTIARWGRTTATLFAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRV
IQGLSMTSGMRATELFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIGTLLVAMYL
PLFNLGNVVA

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=80996 HT085_RS08580 WP_003689811.1 1637903..1639135(+) (pilG) [Neisseria gonorrhoeae strain TUM19854]
ATGGCTAAAAATGGGGGATTTTCTTTGTTCGCAAAGAAAGAAAAACGCTTTATCTTTGAAGGCAGGCATTCTGCCTCCGA
CAAACTGGTCAACGGCGAAGTATCCGCGTTTACCGAAGAAGAGGCACGCAAAAAACTGGCAAAACGCGGCATCCGCCCGT
TGCAGATTACCCGTGTGAAAACAAGCTCCAAGCGCAAAATCACACAAGAAGACATTACCGTTTTCACCCGCCAGCTTTCC
ACGATGATTAAAGCGGGCCTGCCGCTGATGCAGGCATTTGAAATCGTGGCGCGCGGACACGGCAACCCGTCTATGACGGA
AATGCTGATGGAAATCCGAGGCCAAGTGGAACAGGGCAGCTCGTTGAGCCGCGCATTCTCAAACCACCCGAAATATTTCG
ACCGCTTCTACTGCAACCTGGTTGCGGCGGGCGAAACGGGCGGTGTATTGGAAAGCCTGCTGGACAAATTGGCAATTTAC
AAGGAAAAAACCCAGGCCATCCGCAAAAAGGTAAAAACCGCGCTAACCTATCCGGTATCCGTCATCGCCGTCGCCATCGG
TTTGGTATTCGTGATGATGATTTTCGTACTGCCCGCCTTTAAAGAAGTTTACGCCAATATGGGCGCGGAGCTTCCCCCAC
TGACCCAAACAGTGATGGATATGTCCGACTTTTTCGTCTCATACGGCTGGATGGTGCTGATCGCACTGGGCTTTGCCATA
TACGGCTTCCTTAAATTGAAGGCGCGTTCGATTAAAATCCAACGGCGTATGGATGCCATACTGCTGCGTATGCCGATTTT
CGGAGACATTGTCCGCAAAGGGACGATTGCCCGCTGGGGCAGGACGACGGCAACGCTGTTTGCCGCCGGCGTGCCTTTGG
TCGATGTATTGGACTCCACTGCCGGCGCGGCGGGCAATTTAATCTATGAAGAAGCCACCCGGGAAATCCGTACGCGCGTC
ATCCAAGGTCTGTCTATGACTTCGGGGATGCGTGCGACGGAACTGTTCCCCAATATGATGTTGCAGATGTCCTCCATCGG
CGAGGAATCGGGTTCTTTGGACGATATGCTCAACAAAGCCGCCGAATTTTACGAAGACGAGGTGGACAATGCGGTCGGCA
GGCTGTCCGCTATGATGGAGCCGATTATTATTGTGATTTTGGGCTTGGTCATCGGTACGCTTCTGGTCGCTATGTATCTG
CCGCTGTTCAACTTGGGCAACGTGGTCGCCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(281-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

100

100

1
  pilG Neisseria meningitidis 44/76-A

98.78

100

0.988
  pilC Acinetobacter baumannii D1279779

47.236

97.073

0.459
  pilC Acinetobacter baylyi ADP1

45.363

97.317

0.441
  pilC Pseudomonas stutzeri DSM 10701

44.665

98.293

0.439
  pilC Legionella pneumophila strain ERS1305867

44.836

96.829

0.434
  pilC Vibrio cholerae strain A1552

41.294

98.049

0.405
  pilC Vibrio campbellii strain DS40M4

39.163

99.024

0.388

Multiple sequence alignment