Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RPM46_RS10255 Genome accession   NZ_CP135096
Coordinates   2224966..2226459 (-) Length   497 a.a.
NCBI ID   WP_023107818.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain Paer3554     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2219966..2231459
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RPM46_RS10220 (RPM46_10220) - 2220817..2221266 (+) 450 WP_003094369.1 hypothetical protein -
  RPM46_RS10225 (RPM46_10225) - 2221279..2221890 (+) 612 WP_003098862.1 superoxide dismutase -
  RPM46_RS10230 (RPM46_10230) - 2221909..2222841 (+) 933 WP_003120918.1 ZIP family metal transporter -
  RPM46_RS10235 (RPM46_10235) - 2222938..2223210 (-) 273 WP_003094362.1 HPr family phosphocarrier protein -
  RPM46_RS10240 (RPM46_10240) rapZ 2223240..2224100 (-) 861 WP_003094361.1 RNase adapter RapZ -
  RPM46_RS10245 (RPM46_10245) ptsN 2224102..2224566 (-) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  RPM46_RS10250 (RPM46_10250) hpf 2224580..2224888 (-) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  RPM46_RS10255 (RPM46_10255) rpoN/rpoN1 2224966..2226459 (-) 1494 WP_023107818.1 RNA polymerase factor sigma-54 Machinery gene
  RPM46_RS10260 (RPM46_10260) lptB 2226685..2227410 (-) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  RPM46_RS10265 (RPM46_10265) lptA 2227410..2227937 (-) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  RPM46_RS10270 (RPM46_10270) lptC 2227924..2228496 (-) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  RPM46_RS10275 (RPM46_10275) - 2228505..2229044 (-) 540 WP_003098849.1 KdsC family phosphatase -
  RPM46_RS10280 (RPM46_10280) kdsD 2229044..2230024 (-) 981 WP_003094347.1 arabinose-5-phosphate isomerase KdsD -
  RPM46_RS10285 (RPM46_10285) - 2230438..2231247 (+) 810 WP_003094343.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56028.18 Da        Isoelectric Point: 4.4327

>NTDB_id=809422 RPM46_RS10255 WP_023107818.1 2224966..2226459(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain Paer3554]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTAWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=809422 RPM46_RS10255 WP_023107818.1 2224966..2226459(-) (rpoN/rpoN1) [Pseudomonas aeruginosa strain Paer3554]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTACTGCTGCAACTGCGCCAGTTGCCGTCCACCACGGCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATTGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477