Detailed information NTG0809259

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: P3X35_RS05995
• Genome accession: NZ_CP120920
• Coordinates: 1199756..1203448 (+)
• Length: 1230 a.a.
• NCBI ID: WP_059231171.1
• Uniprot ID: -
• Organism: Bacillus paralicheniformis strain SYN-191
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1194756..1208448

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P3X35_RS05985 (P3X35_05985)	modB	1195503..1196159 (+)	657	WP_025811698.1	molybdate ABC transporter permease subunit	-
P3X35_RS05990 (P3X35_05990)	addB	1196269..1199769 (+)	3501	WP_025811696.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
P3X35_RS05995 (P3X35_05995)	addA	1199756..1203448 (+)	3693	WP_059231171.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
P3X35_RS06000 (P3X35_06000)	sbcD	1203514..1204695 (+)	1182	WP_059231169.1	exonuclease subunit SbcD	-
P3X35_RS06005 (P3X35_06005)	sbcC	1204692..1208084 (+)	3393	WP_277868602.1	exonuclease subunit SbcC	-
P3X35_RS06010 (P3X35_06010)	-	1208129..1208347 (-)	219	WP_016885923.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141233.07 Da
• Isoelectric Point: 5.4195

>NTDB_id=809259 P3X35_RS05995 WP_059231171.1 1199756..1203448(+) (addA) [Bacillus paralicheniformis strain SYN-191]
MEISKPKGSNWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITDQEHPVDVDRLLVVTFTNASAAEMKHRIGEA
LEKELAENPGSLHLRRQLALLNKASISTLHSFCLQVIRKYYYLIDVDPAFRIADQTEGELLGDEVLDELFEEEYKKQDPA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPEGWLHTFVDLYDAASDTKVEALPFYSYIKEDIALVLEGMRQKLTRA
LDLTKQPGGPAPRAENFLDDLAQIDRLIQQQNDFSALYELVPAVSFQRLKPCKGDAYDPRLVDEAADLRNSAKKQLEKLK
SDYFTRTPEQHLESLREMKPVIQTLVQLVLEYGKRFADAKKEKAIVDFSDLEHDCLAILSGKNQAGEAVPSEAAKFYRHQ
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLAKYKRFTDDGSGSGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVEYDEQAELKLGASYPSNEATKTELLLIENQSGAQDEEAEELEAVQLEARAMAGQIRRL
ITEKFQVYDAKAKASRNIQYRDIVVLLRSMPWAPQIMDEFKQQGIPVYANLSTGYFEATEVSVTLSLLKIIDNAYQDIPL
ASVLRSPVVGLDENELSLIRIKDKKAPFYEAMKAYLAAADGDERLSEKLRRFDALLKKWRAYAKNHSVAELIWEIYRDTK
YLDYVGGMPGGKQRQANLRALYDRARSYEATSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLIAMKKKLRRELLSEELRVLYVALTRAKEKLYLVG
TAKDKEKLLADWRSQAAGSEWLLPDYERFQAKSYLDFIGPALMRHRDMDESGAPLVIDEIREHPARFQVSWLSAADLQAE
DIDQAGEERHGRLSQIQMGLPIDGSFEYEQQVRERLTWSYPYKDAAKVRTKQSVSEMKRQKEYEDEYGDRSLIRPTKDAL
LFKRPSFMMAKGLTASERGTAMHTVMQHIPLTRALEMNELSRLLDRLVEKELLTEDQRAAIEEDDILAFFDTEIGQKLLG
ARRVEREVPFNMTLSAKEVYPDLESADEPVLIQGIIDCLFETEDGFYLLDYKTDRIHGKYRNGFEGAEPILRKRYETQIQ
LYARAVETMIKMPLKGRALYFFDGGHVLLF

Nucleotide

• Length: 3693 bp

>NTDB_id=809259 P3X35_RS05995 WP_059231171.1 1199756..1203448(+) (addA) [Bacillus paralicheniformis strain SYN-191]
ATGGAGATTTCTAAACCGAAGGGCAGCAATTGGACTGATGACCAATGGAAGGCGATCGTCTCATCCGGCCGGGACATTTT
AGTTGCGGCAGCGGCCGGCTCCGGAAAAACGGCCGTGCTGGTTGAGCGGATCATTCGGAAAATAACGGATCAGGAACATC
CCGTAGATGTCGACAGGCTGCTTGTCGTCACCTTTACGAATGCATCTGCGGCTGAAATGAAGCACAGGATCGGCGAAGCG
CTCGAAAAAGAGCTTGCCGAAAATCCGGGATCGCTTCATTTGCGCAGACAGCTTGCTTTATTAAACAAAGCGAGCATATC
AACATTGCACTCATTCTGTCTTCAAGTCATCCGCAAATACTATTATTTGATCGATGTTGATCCGGCTTTCCGGATCGCCG
ATCAGACGGAAGGCGAGCTGCTTGGCGATGAGGTGCTGGACGAGCTGTTTGAAGAAGAATACAAAAAGCAAGATCCGGCT
TTTTTTGAACTTGTGGACAGATATACGACAGACCGTCATGACCTCGATTTGCAGCATCTCGTCAAGCGGGTCTACGAGTT
TTCCCGTTCGCATCCCGATCCGGAAGGCTGGCTTCACACATTTGTTGATCTGTATGACGCGGCTTCAGATACGAAAGTTG
AGGCTCTGCCGTTTTATTCTTACATTAAAGAAGACATCGCACTTGTTCTGGAGGGGATGCGGCAAAAGCTGACGCGTGCG
CTTGATTTGACAAAGCAGCCAGGGGGGCCTGCGCCGCGCGCCGAAAATTTCCTCGATGATTTAGCGCAGATTGACAGGCT
GATTCAGCAGCAGAATGATTTTTCGGCTCTGTACGAGCTTGTACCGGCCGTTTCGTTTCAAAGATTGAAGCCGTGCAAAG
GAGATGCGTACGATCCTCGATTGGTTGATGAGGCCGCAGATCTCCGCAACAGCGCGAAAAAGCAACTTGAAAAGCTGAAA
AGCGATTATTTTACGCGAACTCCCGAGCAGCACCTTGAGAGCTTGAGGGAAATGAAGCCGGTCATTCAGACGCTCGTCCA
GCTCGTGTTGGAGTATGGCAAGCGGTTTGCGGACGCGAAAAAGGAAAAAGCGATCGTCGATTTCTCAGACCTTGAACACG
ACTGCCTTGCGATTTTATCCGGGAAAAACCAAGCCGGCGAAGCCGTGCCGAGCGAAGCGGCGAAGTTTTACCGCCATCAG
TTCCATGAAGTGCTTGTTGATGAATATCAGGATACGAACTTGGTCCAGGAAGCGATTTTAAAGCTCGTCGCCAAAGAGGA
GCATGAGGGGAACCTGTTTATGGTGGGCGATGTCAAGCAGTCGATTTACCGGTTCCGCCTGGCAGAACCGCTTTTGTTCC
TCGCGAAATATAAACGCTTTACAGACGATGGCTCGGGTTCCGGCCAAAAAATCGATTTAAATAAAAACTTCAGAAGCCGT
TCAGATATTTTGGACAGCACGAACTTTTTATTCAAGCAGCTCATGGGAGAAAAGGTCGGGGAAGTCGAATATGACGAACA
AGCCGAATTGAAGCTTGGCGCGTCTTATCCGTCGAACGAAGCGACAAAAACGGAGCTTCTGCTCATCGAAAACCAGAGCG
GCGCGCAGGATGAAGAGGCTGAAGAGCTTGAGGCGGTCCAGCTTGAAGCAAGAGCGATGGCCGGACAAATCCGCAGGCTG
ATAACAGAAAAATTTCAAGTATATGATGCCAAAGCGAAAGCGTCGCGGAACATCCAGTACCGTGATATCGTGGTGCTTTT
GCGGTCGATGCCTTGGGCGCCGCAAATTATGGACGAGTTTAAACAGCAGGGGATCCCGGTATACGCGAACCTCTCAACGG
GTTATTTTGAAGCGACAGAAGTATCGGTCACACTGTCTTTATTAAAAATCATCGATAATGCTTATCAGGACATCCCGCTT
GCGTCCGTTCTCAGATCGCCTGTTGTAGGTCTTGACGAAAATGAGCTTTCCTTGATCAGAATCAAGGATAAAAAAGCGCC
GTTTTACGAAGCGATGAAAGCATACCTTGCTGCAGCAGACGGCGATGAAAGGCTCAGCGAAAAGCTTCGCCGCTTTGACG
CCCTGCTCAAAAAGTGGAGAGCGTATGCGAAAAACCACTCTGTGGCAGAGCTGATCTGGGAAATCTACCGGGATACGAAG
TATTTGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGCGCTTTACGACAGGGCAAGATC
CTATGAAGCGACATCGTTCCGCGGTTTGTTCCGCTTTTTAAGGTTTATTGAGCGGATGCAGGAGCGGGGTGATGATCTCG
GAACGGCAAGAGCTCTGAGCGAACAGGAAGACGTTGTTCGGCTGATGACGATCCACTCAAGCAAAGGGCTTGAATTTCCT
GTCGTATTTACCGCCGGTCTCGGACGGAATTTTAATATGATGGACTTGAACAAGTCTTATCTGCTGGATAAAGAACTCGG
TTTCGGAACGAAGTTTATTCATCCGAAGTGGCGGATCAGCTATCCGACGCTGCCGCTGATCGCGATGAAGAAAAAGCTCC
GCCGCGAGCTTTTATCAGAGGAGCTGCGCGTCTTGTATGTCGCTCTTACCCGGGCCAAGGAAAAGCTGTACCTGGTCGGG
ACGGCTAAGGATAAAGAAAAGCTGCTTGCCGACTGGCGGTCACAAGCGGCGGGGTCTGAGTGGCTGCTTCCGGATTATGA
ACGGTTTCAGGCGAAATCTTACCTGGATTTCATCGGACCTGCTTTGATGAGACACAGAGATATGGATGAATCCGGCGCGC
CGCTGGTGATTGATGAGATCCGTGAACATCCGGCGCGCTTTCAGGTCAGCTGGCTGTCGGCCGCGGATCTTCAAGCAGAA
GATATTGATCAGGCCGGTGAAGAAAGACACGGCCGGCTCTCCCAAATTCAAATGGGTCTTCCGATCGATGGTTCGTTTGA
GTATGAGCAACAAGTCAGAGAAAGGCTGACATGGTCATATCCATACAAAGACGCCGCCAAAGTCCGAACAAAACAGTCTG
TCTCAGAAATGAAAAGGCAGAAGGAGTATGAGGATGAGTACGGCGACCGCTCGCTGATTCGCCCAACCAAGGATGCTCTC
CTGTTTAAACGGCCGTCCTTTATGATGGCAAAAGGCCTGACCGCTTCCGAACGGGGAACGGCGATGCACACCGTCATGCA
GCATATTCCGCTGACCCGTGCGCTTGAAATGAATGAGCTCAGCCGCCTCCTAGACAGGCTTGTTGAGAAAGAGCTATTAA
CAGAAGACCAGCGGGCAGCGATTGAGGAAGACGATATTCTCGCATTCTTTGATACGGAAATCGGACAAAAGCTTCTCGGC
GCCCGCCGCGTCGAACGGGAAGTTCCCTTTAACATGACGCTGTCCGCCAAAGAGGTTTATCCTGATCTTGAGTCAGCCGA
CGAACCTGTATTGATACAGGGAATTATCGATTGTTTATTCGAGACGGAGGACGGTTTTTACCTTCTTGATTACAAAACCG
ACCGGATACACGGAAAGTACCGAAACGGATTCGAAGGGGCCGAACCGATTTTAAGGAAGCGTTACGAAACACAAATTCAG
CTTTATGCACGAGCCGTTGAAACGATGATTAAAATGCCGCTCAAAGGGCGTGCACTGTACTTTTTTGACGGAGGACATGT
GCTCTTATTTTAA

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1051-1222)

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	71.974	100	0.72