Detailed information NTG0809222

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: P6N31_RS04955
• Genome accession: NZ_CP120905
• Coordinates: 1012526..1013323 (+)
• Length: 265 a.a.
• NCBI ID: WP_015585126.1
• Uniprot ID: A0A855FAW5
• Organism: Raoultella ornithinolytica strain GY22PK023
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1007526..1018323

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P6N31_RS04920 (P6N31_04905)	gspH	1007646..1008164 (+)	519	WP_015585120.1	type II secretion system minor pseudopilin GspH	-
P6N31_RS04925 (P6N31_04910)	gspI	1008161..1008526 (+)	366	WP_004858044.1	type II secretion system minor pseudopilin GspI	-
P6N31_RS04930 (P6N31_04915)	gspJ	1008538..1009122 (+)	585	WP_015585121.1	type II secretion system minor pseudopilin GspJ	-
P6N31_RS04935 (P6N31_04920)	gspK	1009119..1010105 (+)	987	WP_015585122.1	type II secretion system minor pseudopilin GspK	-
P6N31_RS04940 (P6N31_04925)	gspL	1010092..1011294 (+)	1203	WP_204111659.1	type II secretion system protein GspL	-
P6N31_RS04945 (P6N31_04930)	gspM	1011287..1011781 (+)	495	WP_064357507.1	type II secretion system protein GspM	-
P6N31_RS04950 (P6N31_04935)	-	1011771..1012511 (+)	741	WP_015585125.1	type II secretion system protein N	-
P6N31_RS04955 (P6N31_04940)	pilD	1012526..1013323 (+)	798	WP_015585126.1	A24 family peptidase	Machinery gene
P6N31_RS04960 (P6N31_04945)	hrpB	1013344..1015773 (-)	2430	WP_044348317.1	ATP-dependent helicase HrpB	-
P6N31_RS04965 (P6N31_04950)	thpR	1015846..1016382 (+)	537	WP_004858022.1	RNA 2',3'-cyclic phosphodiesterase	-
P6N31_RS04970 (P6N31_04955)	sfsA	1016382..1017098 (+)	717	WP_004858020.1	DNA/RNA nuclease SfsA	-
P6N31_RS04975 (P6N31_04960)	dksA	1017263..1017718 (+)	456	WP_004858018.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29285.93 Da
• Isoelectric Point: 8.4762

>NTDB_id=809222 P6N31_RS04955 WP_015585126.1 1012526..1013323(+) (pilD) [Raoultella ornithinolytica strain GY22PK023]
MTLLSAFSLQFPLLWGGLLFIFGLTFGSFFNVVIYRLPLMMQQEESARFNLCVPASFCPQCQRPIIWRDNIPLLSYLSLK
GRARCCQAPISQRYPLTELASGLLFVLAGYLLTPGLPLLGGLILLSTLLVLAIIDGQTQLLPDRLTLPLLWAGLLFNLNG
TFVPLSEAVIGAMTGYLSLWTVYWLFRLLTGKEALGYGDFKLLAALGAWSGWQILPQTLLCASASGLIWTLLQRRITLQS
LDQPLAFGPWLALAGSGLFLWTQTL

Nucleotide

• Length: 798 bp

>NTDB_id=809222 P6N31_RS04955 WP_015585126.1 1012526..1013323(+) (pilD) [Raoultella ornithinolytica strain GY22PK023]
ATGACGCTACTGAGCGCATTTTCGTTGCAGTTCCCCCTGTTATGGGGCGGATTGTTGTTTATTTTCGGCCTGACGTTTGG
CAGCTTTTTTAACGTGGTTATCTACCGTTTGCCGCTGATGATGCAGCAGGAAGAGTCCGCACGATTCAACCTGTGCGTTC
CCGCCTCCTTCTGCCCCCAGTGCCAGCGGCCCATCATCTGGCGGGATAATATTCCGCTGCTCAGCTATCTCTCACTCAAA
GGGCGCGCCCGCTGCTGCCAGGCGCCCATTTCTCAGCGCTATCCGCTGACGGAACTGGCCAGCGGACTGCTCTTTGTGTT
GGCCGGCTATCTGCTGACGCCGGGATTACCGCTGCTGGGGGGCCTGATCCTGCTCTCCACGCTGCTGGTACTGGCGATCA
TCGACGGCCAGACTCAGCTCCTGCCGGATCGGCTGACTCTGCCGCTGCTGTGGGCCGGTCTGCTGTTTAATCTCAACGGC
ACGTTTGTCCCGCTCAGCGAAGCGGTGATCGGCGCCATGACGGGCTATTTATCACTGTGGACGGTTTACTGGCTGTTTCG
CCTGCTGACCGGCAAAGAGGCGCTAGGATACGGTGATTTTAAGCTGCTGGCGGCACTCGGCGCATGGTCCGGCTGGCAGA
TCCTGCCGCAGACGCTGCTGTGCGCCTCGGCCAGCGGCCTGATCTGGACCTTGCTGCAACGGCGGATCACGCTCCAGTCG
CTGGATCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCAGCGGCCTGTTTCTGTGGACTCAGACGCTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (122-230)

3D structure

• AlphaFold DB: A0A855FAW5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.529	100	0.498
pilD	Vibrio campbellii strain DS40M4	45.221	100	0.464
pilD	Neisseria gonorrhoeae MS11	46.614	94.717	0.442
pilD	Acinetobacter baumannii D1279779	45.276	95.849	0.434
pilD	Acinetobacter nosocomialis M2	44.186	97.358	0.43