Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   OO978_RS02085 Genome accession   NZ_AP026783
Coordinates   449618..450295 (-) Length   225 a.a.
NCBI ID   WP_001377644.1    Uniprot ID   -
Organism   Escherichia coli strain IPCEC31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444618..455295
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO978_RS02070 (IPCEC31_04120) chiA 445985..448678 (+) 2694 WP_001377643.1 bifunctional chitinase/lysozyme -
  OO978_RS02075 (IPCEC31_04130) bfd 448847..449041 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  OO978_RS02080 (IPCEC31_04140) bfr 449113..449589 (+) 477 WP_000675504.1 bacterioferritin -
  OO978_RS02085 (IPCEC31_04150) pilD 449618..450295 (-) 678 WP_001377644.1 prepilin peptidase GspO Machinery gene
  OO978_RS02090 (IPCEC31_04160) gspM 450295..450756 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  OO978_RS02095 (IPCEC31_04170) gspL 450753..451916 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  OO978_RS02100 (IPCEC31_04180) gspK 451931..452914 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  OO978_RS02105 (IPCEC31_04190) gspJ 452907..453494 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  OO978_RS02110 (IPCEC31_04200) gspI 453487..453864 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  OO978_RS02115 (IPCEC31_04210) gspH 453861..454370 (-) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  OO978_RS02120 (IPCEC31_04220) gspG 454378..454815 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.75 Da        Isoelectric Point: 7.8986

>NTDB_id=80913 OO978_RS02085 WP_001377644.1 449618..450295(-) (pilD) [Escherichia coli strain IPCEC31]
MTMLLPLFILVGFIAGCFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=80913 OO978_RS02085 WP_001377644.1 449618..450295(-) (pilD) [Escherichia coli strain IPCEC31]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTGTTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382
  pilD Vibrio cholerae strain A1552

35.745

100

0.373

Multiple sequence alignment