Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   P2A85_RS28065 Genome accession   NZ_CP120853
Coordinates   6448729..6450732 (-) Length   667 a.a.
NCBI ID   WP_377476745.1    Uniprot ID   -
Organism   MAG: Microcoleus anatoxicus isolate Neu2020_A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6443729..6455732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P2A85_RS28040 (P2A85_28010) - 6444326..6445162 (-) 837 WP_377476741.1 helix-turn-helix domain-containing protein -
  P2A85_RS28045 (P2A85_28015) - 6445180..6445794 (-) 615 WP_377476742.1 hypothetical protein -
  P2A85_RS28050 (P2A85_28020) - 6446071..6447198 (-) 1128 WP_340523884.1 type II secretion system F family protein -
  P2A85_RS28055 (P2A85_28025) - 6447339..6447500 (-) 162 WP_377476743.1 hypothetical protein -
  P2A85_RS28060 (P2A85_28030) - 6447478..6448572 (-) 1095 WP_377476744.1 type IV pilus twitching motility protein PilT -
  P2A85_RS28065 (P2A85_28035) pilB/pilB1 6448729..6450732 (-) 2004 WP_377476745.1 GspE/PulE family protein Machinery gene
  P2A85_RS28070 (P2A85_28040) grpE 6451580..6452374 (+) 795 WP_377476746.1 nucleotide exchange factor GrpE -
  P2A85_RS28075 (P2A85_28045) dnaK 6452570..6454708 (+) 2139 WP_377476747.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74519.52 Da        Isoelectric Point: 4.9747

>NTDB_id=808924 P2A85_RS28065 WP_377476745.1 6448729..6450732(-) (pilB/pilB1) [MAG: Microcoleus anatoxicus isolate Neu2020_A]
MTNTSDRRFSTSIVTISNFTPALNKMIQSGYVNSDQLKKAQLESRKSGKRLTEVLESATGLPLPPELLRLYKKQQMFELK
IVYGVEAFDPELSELSNEQLGAMLDELKMSIDICRQRRFLPVAKTETEPPALVVAMVDPDNLDAQEDLRRILKGWSFQRR
VITPDDYQDITSAYQEQQVKIAAKIAEAEKERKLSLGEGDFGELSLEEVSSDQEDIADTINAVEQAPVIKAVNVILAKAL
SEKVSDIHVEPQEEFMRIRMRKDGVLQEYFRFPKQVVQGITARFKIIANLDIAERRQAQDGRIRRVFEGRTVDFRVNSLP
SRYGEKIVLRILDSSSTQLGLGLLISDPETLALVRETASRPFGLILVTGPTGSGKSTSLYSILAEKNDPAINICTAEDPI
EYALPGITQVQVIREKDLTFSNILRAFLRQDPDVMLVGETRDKETAKTALEAALTGHLVLTTLHTNDAAGAIARLDEMGV
EPFMVSAALLGVLAQRLMRRVCDKCYIPYQPTALELGRYGLSATQEVDLTVYKANTIPVEQRKELAEQDQLCKKCGGIGY
KGRVGVYEFLKNTERLQTLITQGAPTEQIKEAAVEEGMTTLLAYSLQLVREGATTFEEVERVTFTDSGLEAEMKAKRKQG
LTCKTCQAELKPEMLDCPYCLTPRFLD

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=808924 P2A85_RS28065 WP_377476745.1 6448729..6450732(-) (pilB/pilB1) [MAG: Microcoleus anatoxicus isolate Neu2020_A]
ATGACTAACACATCCGATCGGCGTTTCTCCACATCTATAGTTACTATCAGCAATTTTACTCCGGCTCTCAACAAGATGAT
TCAGTCTGGTTACGTCAACAGCGACCAACTGAAAAAAGCTCAACTTGAAAGTCGGAAGTCAGGTAAGCGGTTGACGGAGG
TACTAGAATCTGCTACAGGGCTACCCTTGCCGCCAGAATTGCTGCGGCTTTACAAAAAGCAGCAGATGTTTGAACTCAAA
ATAGTGTACGGGGTTGAAGCGTTTGACCCGGAATTGAGCGAACTGTCTAACGAGCAACTTGGAGCAATGCTCGATGAGTT
GAAAATGTCGATCGACATCTGTCGTCAACGTCGGTTTTTGCCTGTGGCGAAGACAGAAACTGAACCTCCGGCGCTGGTAG
TGGCAATGGTCGATCCTGACAACCTGGATGCTCAAGAAGATTTGCGACGGATTTTGAAGGGGTGGAGTTTTCAGCGGCGG
GTGATTACCCCAGATGACTATCAGGACATTACTTCTGCCTATCAAGAACAGCAAGTTAAGATAGCAGCCAAAATAGCTGA
AGCCGAAAAGGAGAGAAAGCTCTCCCTAGGGGAGGGAGATTTTGGCGAACTGTCCTTAGAAGAGGTTTCTTCCGATCAAG
AAGATATAGCTGACACCATAAACGCTGTAGAGCAAGCTCCTGTAATCAAAGCGGTCAACGTCATTTTGGCTAAAGCTTTA
TCGGAAAAAGTCTCTGACATCCACGTAGAACCACAAGAAGAGTTCATGCGAATTCGGATGCGGAAGGATGGGGTGCTGCA
AGAGTATTTCCGATTTCCCAAGCAAGTGGTGCAAGGGATTACTGCTAGGTTCAAAATTATTGCCAATTTGGACATTGCCG
AACGCAGACAAGCTCAAGATGGTCGCATCCGTCGGGTATTTGAAGGGCGGACGGTGGATTTCCGGGTAAATAGCTTGCCT
TCCCGCTACGGCGAAAAAATTGTGTTGCGGATTTTGGATAGTTCCAGCACTCAGTTGGGTCTGGGTTTGTTAATCTCCGA
TCCAGAAACTTTGGCTTTGGTTCGAGAAACTGCCAGCCGCCCCTTTGGACTGATTTTGGTGACTGGGCCGACTGGTTCGG
GTAAGTCAACTTCGCTGTATTCAATTCTGGCGGAAAAGAATGATCCGGCGATTAACATTTGTACGGCGGAAGACCCGATC
GAATATGCTTTGCCGGGAATTACCCAGGTCCAAGTAATTCGGGAAAAAGACTTGACTTTTTCCAACATTCTGCGGGCGTT
TTTGCGGCAAGACCCTGACGTTATGCTAGTGGGGGAAACGCGGGACAAGGAAACGGCGAAAACGGCTCTGGAAGCTGCTT
TGACCGGACACTTGGTATTGACGACGCTGCACACAAACGACGCGGCAGGTGCGATCGCCCGTTTGGACGAAATGGGCGTA
GAACCGTTCATGGTGTCGGCGGCTTTGTTGGGTGTGTTGGCTCAGCGTCTGATGCGGCGCGTTTGTGACAAATGCTACAT
TCCTTATCAGCCTACTGCGCTCGAACTCGGTCGCTACGGCCTGTCAGCTACTCAAGAAGTGGATCTGACTGTCTACAAAG
CCAACACCATACCAGTTGAGCAGAGGAAGGAACTGGCAGAACAGGACCAACTGTGTAAGAAGTGCGGAGGCATAGGCTAC
AAAGGGCGGGTAGGGGTTTACGAATTCCTGAAAAACACTGAGCGGCTGCAAACTCTGATCACTCAAGGTGCTCCCACAGA
GCAAATTAAGGAAGCCGCAGTAGAAGAAGGGATGACAACCTTGCTGGCTTACAGTTTGCAGTTAGTGCGGGAAGGCGCGA
CTACTTTTGAGGAAGTCGAGCGGGTGACGTTTACAGATTCTGGGCTGGAGGCTGAAATGAAGGCAAAACGCAAGCAGGGG
CTGACCTGCAAGACTTGTCAGGCAGAATTGAAGCCGGAGATGCTGGATTGTCCGTACTGTTTGACTCCGCGCTTTCTAGA
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(235-500)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

60

98.201

0.589