Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   P4829_RS13465 Genome accession   NZ_CP120846
Coordinates   2664294..2664917 (-) Length   207 a.a.
NCBI ID   WP_010789256.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain MBLB1156     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659294..2669917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4829_RS13435 (P4829_13435) rpsT 2659588..2659854 (+) 267 WP_003325396.1 30S ribosomal protein S20 -
  P4829_RS13440 (P4829_13440) holA 2659869..2660912 (-) 1044 WP_063638566.1 DNA polymerase III subunit delta -
  P4829_RS13445 (P4829_13445) - 2660952..2661101 (-) 150 WP_010789254.1 hypothetical protein -
  P4829_RS13450 (P4829_13450) - 2661142..2661276 (+) 135 WP_003229983.1 YqzM family protein -
  P4829_RS13455 (P4829_13455) comEC 2661317..2663650 (-) 2334 WP_063638567.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  P4829_RS13460 (P4829_13460) - 2663657..2664226 (-) 570 WP_003325392.1 ComE operon protein 2 -
  P4829_RS13465 (P4829_13465) comEA 2664294..2664917 (-) 624 WP_010789256.1 helix-hairpin-helix domain-containing protein Machinery gene
  P4829_RS13470 (P4829_13470) comER 2665001..2665822 (+) 822 WP_063638568.1 late competence protein ComER -
  P4829_RS13475 (P4829_13475) - 2665892..2666632 (-) 741 WP_063638569.1 class I SAM-dependent methyltransferase -
  P4829_RS13480 (P4829_13480) rsfS 2666629..2666985 (-) 357 WP_003325387.1 ribosome silencing factor -
  P4829_RS13485 (P4829_13485) yqeK 2667002..2667562 (-) 561 WP_010789259.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  P4829_RS13490 (P4829_13490) - 2667552..2668121 (-) 570 WP_003325385.1 nicotinate-nucleotide adenylyltransferase -
  P4829_RS13495 (P4829_13495) yhbY 2668133..2668423 (-) 291 WP_003325384.1 ribosome assembly RNA-binding protein YhbY -
  P4829_RS13500 (P4829_13500) aroE 2668417..2669259 (-) 843 WP_061669959.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 21961.72 Da        Isoelectric Point: 4.6181

>NTDB_id=808861 P4829_RS13465 WP_010789256.1 2664294..2664917(-) (comEA) [Bacillus atrophaeus strain MBLB1156]
MNWFYQHKKIILTAGAAVIFIVILLNFPGAKKQEPVQQITASPDTAGTQVQQQESKGDADGEKIIIDIKGAVKHPGVYEL
KTGDRVSQAIEKAGGINSEADEKQVNLAELLQDGTVVYIPSEGEEPTQSAAANASVQNGAGKEALVNINTASLEELQAIS
GVGPSKAEAIIAYREENGKFQAVEDITNVSGIGEKSFEKIKSAITVK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=808861 P4829_RS13465 WP_010789256.1 2664294..2664917(-) (comEA) [Bacillus atrophaeus strain MBLB1156]
ATGAATTGGTTTTACCAGCACAAAAAAATAATCTTAACGGCAGGTGCTGCTGTCATCTTCATCGTCATCCTGCTTAATTT
TCCCGGCGCAAAGAAGCAGGAGCCTGTTCAGCAAATCACAGCGTCGCCTGACACGGCGGGCACACAAGTTCAGCAGCAAG
AATCGAAAGGTGATGCTGACGGAGAAAAAATTATCATTGATATTAAAGGCGCTGTCAAACATCCGGGTGTTTACGAATTG
AAAACGGGAGACAGAGTTTCACAGGCTATCGAAAAAGCGGGAGGCATTAATTCGGAGGCTGATGAAAAACAAGTAAATTT
AGCTGAACTCCTGCAGGACGGCACTGTCGTGTACATTCCGAGTGAGGGGGAAGAGCCAACACAAAGCGCTGCCGCAAATG
CTTCTGTGCAAAACGGAGCAGGAAAAGAGGCTTTGGTTAATATCAATACAGCGTCATTAGAGGAACTGCAGGCTATTTCC
GGGGTCGGTCCGTCAAAAGCAGAAGCTATAATAGCTTATCGTGAAGAAAACGGAAAATTTCAAGCCGTTGAAGACATTAC
AAATGTCTCGGGAATCGGCGAAAAATCATTTGAGAAAATAAAATCAGCCATCACAGTTAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(66-120)

Helix-hairpin-helix motif (HHH_3)

(143-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

65.7

100

0.657