Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P4831_RS11200 Genome accession   NZ_CP120842
Coordinates   2201664..2203025 (-) Length   453 a.a.
NCBI ID   WP_003130563.1    Uniprot ID   -
Organism   Lactococcus lactis strain ZFM559     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2196664..2208025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4831_RS11165 - 2197213..2197707 (+) 495 WP_003129897.1 GNAT family N-acetyltransferase -
  P4831_RS11170 - 2197799..2198122 (-) 324 WP_003129893.1 DUF1048 domain-containing protein -
  P4831_RS11175 - 2198126..2198455 (-) 330 WP_031560560.1 DUF1048 domain-containing protein -
  P4831_RS11180 - 2198442..2198804 (-) 363 WP_003129889.1 PadR family transcriptional regulator -
  P4831_RS11185 - 2198924..2199553 (-) 630 WP_003129888.1 hypothetical protein -
  P4831_RS11190 - 2199618..2200703 (-) 1086 WP_003129885.1 PIN/TRAM domain-containing protein -
  P4831_RS11195 - 2200934..2201620 (+) 687 WP_012898598.1 YoaK family protein -
  P4831_RS11200 radA 2201664..2203025 (-) 1362 WP_003130563.1 DNA repair protein RadA Machinery gene
  P4831_RS11205 - 2203187..2203414 (-) 228 WP_010906294.1 heavy-metal-associated domain-containing protein -
  P4831_RS11215 rplQ 2204229..2204609 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  P4831_RS11220 - 2204628..2205566 (-) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  P4831_RS11225 rpsK 2205614..2205997 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  P4831_RS11230 rpsM 2206018..2206383 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  P4831_RS11235 rpmJ 2206401..2206517 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  P4831_RS11240 infA 2206571..2206789 (-) 219 WP_003130554.1 translation initiation factor IF-1 -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49893.28 Da        Isoelectric Point: 5.1748

>NTDB_id=808791 P4831_RS11200 WP_003130563.1 2201664..2203025(-) (radA) [Lactococcus lactis strain ZFM559]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=808791 P4831_RS11200 WP_003130563.1 2201664..2203025(-) (radA) [Lactococcus lactis strain ZFM559]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTCGAATTATTTGATACTCCTCGGATTGAAACGGACCTAGATGAATTTAATCGTGTA
TTAGGAGGGGGCGTCGTTCCTGGTTCATTAGTTCTGATTGGGGGAGACCCTGGGATAGGTAAATCAACCTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTCATG
TGACTAAAGAAGGACAGCTGGCTGGTCCAAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGCTCAACTAATGAAATTGGAATCTTTGAGATGCAGGG
TCATGGCTTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGTTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCTATACTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.236

100

0.812
  radA Streptococcus pneumoniae D39

81.236

100

0.812
  radA Streptococcus pneumoniae R6

81.236

100

0.812
  radA Streptococcus pneumoniae TIGR4

81.236

100

0.812
  radA Streptococcus mitis SK321

81.236

100

0.812
  radA Streptococcus mitis NCTC 12261

81.015

100

0.81
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62