Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   P3T49_RS12860 Genome accession   NZ_CP120723
Coordinates   2563683..2564720 (-) Length   345 a.a.
NCBI ID   WP_277713604.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain NX-12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2558683..2569720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T49_RS12810 tasA 2558840..2559622 (-) 783 WP_063638541.1 biofilm matrix protein TasA -
  P3T49_RS12815 sipW 2559686..2560258 (-) 573 WP_010789195.1 signal peptidase I SipW -
  P3T49_RS12820 tapA 2560242..2560943 (-) 702 WP_151290438.1 amyloid fiber anchoring/assembly protein TapA -
  P3T49_RS12825 - 2561205..2561528 (+) 324 WP_088117568.1 DUF3889 domain-containing protein -
  P3T49_RS12830 - 2561575..2561754 (-) 180 WP_003325435.1 YqzE family protein -
  P3T49_RS12835 comGG 2561824..2562198 (-) 375 WP_088117569.1 competence type IV pilus minor pilin ComGG Machinery gene
  P3T49_RS12840 comGF 2562199..2562705 (-) 507 WP_274379311.1 competence type IV pilus minor pilin ComGF -
  P3T49_RS12845 comGE 2562608..2562955 (-) 348 WP_088117570.1 competence type IV pilus minor pilin ComGE Machinery gene
  P3T49_RS12850 comGD 2562939..2563379 (-) 441 WP_088117571.1 competence type IV pilus minor pilin ComGD Machinery gene
  P3T49_RS12855 comGC 2563369..2563668 (-) 300 WP_003325429.1 competence type IV pilus major pilin ComGC Machinery gene
  P3T49_RS12860 comGB 2563683..2564720 (-) 1038 WP_277713604.1 competence type IV pilus assembly protein ComGB Machinery gene
  P3T49_RS12865 comGA 2564707..2565777 (-) 1071 WP_063638548.1 competence type IV pilus ATPase ComGA Machinery gene
  P3T49_RS12870 - 2566179..2567132 (-) 954 WP_088117575.1 magnesium transporter CorA family protein -
  P3T49_RS12875 - 2567254..2568602 (+) 1349 Protein_2514 hemolysin family protein -
  P3T49_RS12880 - 2568673..2569524 (-) 852 WP_277713607.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 40096.30 Da        Isoelectric Point: 9.6994

>NTDB_id=808371 P3T49_RS12860 WP_277713604.1 2563683..2564720(-) (comGB) [Bacillus atrophaeus strain NX-12]
MKRIKKSWPINDQANLLNRLGDMTENGYNLLDGLRLMELQMNKRQRTEMSAAVNRLREGEPFYQVLQALAFHKEAVSICY
LAEMHGELSVAMRQSGGLLERKLTQANQLKKVIRYPIFLIFTVFVMFYILQAVIIPQFSGVYQSMNMETSRTTAVIFAFF
QHVHFAVIGFVLLALGAALYYWFGFKKKSPADKMLFFVRIPILGRLVILFNSYFFSLQLSSLLKSGLSIYDSLKAFKEQS
FLPFYRQEAEQIINQLRAGESIDRALYKHPCYENDFCEVVLHGQLSGQLHRELFTYSQFLMERLERKVEKWTGILQPVIY
GIVAGMILIVYLSMLLPMYQMMNQL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=808371 P3T49_RS12860 WP_277713604.1 2563683..2564720(-) (comGB) [Bacillus atrophaeus strain NX-12]
ATGAAGAGGATTAAAAAAAGCTGGCCGATAAACGATCAAGCTAATTTGCTAAACAGACTCGGTGACATGACAGAAAACGG
ATACAATCTTCTGGATGGATTGCGGTTAATGGAACTGCAAATGAATAAGCGTCAGCGTACGGAAATGTCTGCTGCTGTAA
ATCGTTTACGAGAGGGGGAACCATTCTATCAGGTTTTGCAGGCTTTGGCTTTCCACAAGGAAGCCGTAAGCATTTGTTAT
TTAGCAGAAATGCATGGAGAATTGTCCGTTGCTATGAGGCAAAGCGGAGGGCTGTTGGAAAGGAAATTAACACAGGCCAA
TCAGCTGAAAAAAGTCATCCGTTATCCGATTTTCCTGATCTTCACTGTCTTTGTGATGTTTTATATCCTGCAAGCTGTCA
TCATCCCGCAATTTTCAGGAGTTTATCAGTCCATGAATATGGAAACCTCACGCACAACCGCTGTTATATTCGCTTTTTTT
CAGCACGTTCATTTTGCAGTGATTGGTTTTGTCTTGCTTGCTCTCGGAGCGGCGCTCTATTACTGGTTTGGATTTAAGAA
GAAGTCCCCGGCAGACAAAATGCTCTTTTTTGTACGGATACCCATACTCGGAAGACTTGTCATTTTATTTAACAGCTACT
TTTTTTCTTTGCAGCTCAGCAGCCTATTAAAGTCTGGACTGTCTATTTACGACAGCCTTAAGGCCTTTAAAGAGCAATCG
TTCCTCCCTTTTTACCGTCAGGAGGCAGAACAAATCATTAACCAATTAAGGGCTGGAGAGTCCATAGATAGAGCTCTGTA
TAAACACCCATGCTATGAAAATGACTTTTGTGAAGTGGTGTTGCACGGCCAATTAAGCGGGCAGCTGCATCGTGAACTAT
TCACCTACAGCCAGTTTTTAATGGAGCGGCTGGAGCGAAAGGTTGAAAAATGGACGGGAATCCTTCAGCCCGTCATCTAC
GGTATTGTTGCCGGGATGATTTTAATTGTGTATTTGTCGATGCTTTTGCCTATGTATCAGATGATGAATCAATTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

69.35

93.623

0.649