Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OO986_RS01990 Genome accession   NZ_AP026552
Coordinates   422912..424636 (+) Length   574 a.a.
NCBI ID   WP_011079397.1    Uniprot ID   A0A3Q0L3L1
Organism   Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417912..429636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO986_RS01965 (VV208B2_03480) gspM 418316..418966 (+) 651 WP_045622003.1 type II secretion system protein GspM -
  OO986_RS01970 (VV208B2_03490) - 418959..419279 (+) 321 WP_011079393.1 MSHA biogenesis protein MshK -
  OO986_RS01975 (VV208B2_03500) mshL 419295..420941 (+) 1647 WP_072612424.1 pilus (MSHA type) biogenesis protein MshL -
  OO986_RS01980 (VV208B2_03510) - 420949..421797 (+) 849 WP_103277912.1 ExeA family protein -
  OO986_RS01985 (VV208B2_03520) - 421794..422915 (+) 1122 WP_108695145.1 tetratricopeptide repeat protein -
  OO986_RS01990 (VV208B2_03530) pilF 422912..424636 (+) 1725 WP_011079397.1 GspE/PulE family protein Machinery gene
  OO986_RS01995 (VV208B2_03540) - 424646..425866 (+) 1221 WP_108694838.1 type II secretion system F family protein -
  OO986_RS02000 - 425870..426331 (+) 462 WP_072612421.1 MSHA biogenesis protein MshF -
  OO986_RS02005 (VV208B2_03560) - 426410..426988 (+) 579 WP_011079400.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OO986_RS24485 (VV208B2_03570) - 427022..427477 (+) 456 WP_011079401.1 type II secretion system protein -
  OO986_RS02015 (VV208B2_03580) - 427530..428015 (+) 486 WP_072612420.1 type II secretion system protein -
  OO986_RS02020 (VV208B2_03590) - 428015..428581 (+) 567 WP_011079403.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OO986_RS02025 (VV208B2_03600) - 428581..429270 (+) 690 WP_011079404.1 PilW family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63672.03 Da        Isoelectric Point: 5.3226

>NTDB_id=80833 OO986_RS01990 WP_011079397.1 422912..424636(+) (pilF) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
MKVRLRKRLGDLLVEEGIINEAQLDQALGAQRSGGRKLGDTLIDLGFLSEQQMLAFLSQQLDLPLIDLNRATVDIDAVQL
LPEVHARRLRALVIGRNGDTLRIAMSDPADLFAQEALIGQLGQYGIEFVIAPERQLVEGFDRYYRRTKEIANFAEQLQAE
HQVNDAFDFNIEDDDSDEVTVVKLINSMFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLNEVNIAPALVLRLKLM
ANLDISEKRLPQDGRFNIRSKGQSVDIRMSTMPVQYGESVVMRLLNQSSGVRKLEDSGLPDDLLVRLRRQLKRPHGMILV
TGPTGSGKTTTLYGALTELNQPGKKIITAEDPVEYRLSRVNQVQINPKINLDFSTVLRTFLRQDPDIILIGEMRDHETVE
IGLRAALTGHLVLSTLHTNDAVDSALRMIDMGAPGYLVASAVRAVVAQRLVRRVCPDCKTEDHVGESRKAWLAQRFPNQV
EAGFVKGRGCQNCNLTGYRGRIGVFEMLELEHGMMDALRANDAVEFAHAARRSPGYKPLLASAMELALQGVVSLDEVMNL
GEGDSSGVAEAIIM

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=80833 OO986_RS01990 WP_011079397.1 422912..424636(+) (pilF) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
ATGAAAGTAAGATTAAGAAAGCGACTTGGTGACCTTCTTGTTGAGGAAGGCATTATCAATGAAGCGCAACTTGATCAAGC
ACTCGGCGCGCAAAGAAGTGGTGGACGCAAGCTTGGCGATACGCTGATTGATTTGGGTTTTTTGTCTGAACAACAGATGC
TGGCGTTTTTGTCGCAGCAACTTGATCTGCCTTTAATTGACTTAAATCGAGCCACGGTCGATATCGATGCAGTGCAGTTG
CTACCAGAAGTGCATGCTCGTCGTTTACGCGCATTGGTGATTGGCCGTAATGGCGACACGTTGCGCATTGCGATGAGTGA
TCCTGCGGACCTTTTTGCACAAGAAGCGTTGATCGGTCAGCTAGGACAATATGGCATTGAGTTTGTTATCGCGCCAGAAA
GGCAATTGGTGGAAGGTTTTGACCGCTATTATCGCCGTACCAAAGAGATTGCCAACTTTGCCGAGCAGTTGCAAGCGGAA
CACCAAGTCAACGATGCATTCGATTTCAATATCGAAGATGACGACAGTGATGAGGTGACGGTTGTTAAGCTCATCAACTC
GATGTTTGAAGACGCCATTCAGGTGGGGGCGTCGGATATCCATATCGAACCAGACGCCAATGTGTTGCGTTTGCGCCAAC
GTATTGATGGCGTGTTGCACGAAACCTTGCTCAATGAAGTCAACATCGCGCCAGCCTTGGTACTGAGATTGAAACTCATG
GCCAACTTGGATATTTCAGAAAAGCGTCTGCCACAAGATGGCCGTTTTAACATCCGCTCCAAAGGTCAGTCGGTGGATAT
CCGTATGTCGACCATGCCAGTTCAGTATGGTGAGTCGGTGGTAATGCGTTTGCTCAATCAATCTTCCGGAGTCAGAAAAT
TAGAAGACTCCGGTTTGCCGGACGATCTTTTAGTGCGTCTGCGCCGACAGTTGAAACGTCCGCATGGCATGATCCTTGTT
ACTGGCCCTACGGGATCCGGTAAAACCACCACGCTATATGGTGCTCTAACAGAATTAAACCAACCGGGGAAAAAGATCAT
CACAGCGGAAGATCCGGTTGAATACCGACTGTCGCGTGTCAACCAAGTGCAGATCAATCCGAAGATCAACCTTGATTTTT
CCACCGTGTTAAGAACATTCCTCCGTCAGGATCCCGATATCATTTTGATTGGTGAGATGCGTGACCATGAAACGGTTGAA
ATTGGCCTGCGAGCGGCGCTGACCGGTCACTTAGTATTGAGTACCTTACACACCAATGACGCCGTAGACAGTGCGCTTCG
TATGATTGATATGGGTGCTCCGGGCTATCTGGTCGCGAGTGCCGTACGTGCTGTGGTCGCGCAGCGATTGGTGCGCCGTG
TCTGTCCGGATTGCAAGACAGAAGATCATGTTGGTGAGTCACGCAAAGCGTGGTTGGCACAACGCTTCCCGAATCAAGTG
GAAGCAGGCTTTGTGAAAGGGCGCGGTTGTCAAAACTGTAACTTAACAGGTTATCGCGGCCGTATTGGTGTATTTGAAAT
GCTGGAACTTGAGCATGGAATGATGGACGCGTTGCGCGCCAATGATGCCGTCGAGTTTGCACATGCTGCGCGTCGTTCGC
CTGGCTATAAACCTCTGTTAGCGTCGGCAATGGAGTTGGCGCTGCAAGGCGTAGTGAGCTTAGACGAAGTGATGAATTTA
GGCGAAGGTGACTCCTCTGGGGTTGCTGAAGCGATCATTATGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-148)

Type II/IV secretion system protein (T2SSE)

(187-453)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

38.104

97.387

0.371
  pilB Vibrio cholerae strain A1552

37.699

98.432

0.371

Multiple sequence alignment